Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | competitive versus S-adenosyl-L-methionine | Neurospora crassa | |
S-adenosyl-L-methionine | - |
Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
CNBr peptide | residue 1-80 of horse heart cytochrome c | Neurospora crassa | |
0.03 | - |
[cytochrome c]-L-lysine | of horse heart, heme-free | Neurospora crassa | |
0.04 | - |
CNBr peptide | residue 1-65 or residue 66-104 of horse heart cytochrome c | Neurospora crassa | |
0.2 | - |
[cytochrome c]-L-lysine | of horse heart, ethanol-denatured | Neurospora crassa | |
0.32 | - |
[cytochrome c]-L-lysine | of horse heart, native | Neurospora crassa | |
1.7 | - |
[cytochrome c]-L-lysine | - |
Neurospora crassa | |
19 | - |
S-adenosyl-L-methionine | - |
Neurospora crassa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Neurospora crassa | 5829 | - |
additional information | overview | Neurospora crassa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytochrome c L-lysine | Neurospora crassa | - |
S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0295 | - |
purified enzyme | Neurospora crassa |
Storage Stability | Organism |
---|---|
0-4°C, 50% saturated ammonium sulfate, stable for several months | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + CNBr peptide | - |
Neurospora crassa | ? | - |
? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | - |
Neurospora crassa | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | horse heart cytochrome c-72 | Neurospora crassa | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | a single lysine residue at position 72 of horse heart cytochrome c is methylated | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | horse heart cytochrome c-72 | Neurospora crassa | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | also CNBr peptides of horse heart cytochrome c can serve as substrates, overview | Neurospora crassa | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | a single lysine residue at position 72 of horse heart cytochrome c is methylated | ? | |
S-adenosyl-L-methionine + cytochrome c L-lysine | also CNBr peptides of horse heart cytochrome c can serve as substrates, overview | Neurospora crassa | S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine | mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 | ? | |
S-adenosyl-L-methionine + [cytochrome c]-L-lysine | - |
Neurospora crassa | S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Neurospora crassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Neurospora crassa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
S-adenosyl-L-methionine | - |
Neurospora crassa |