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Literature summary for 2.1.1.59 extracted from

  • Nochumson, S.; Durban, E.; Kim, S.; Paik, W.K.
    Cytochrome c-specific protein methylase III from Neurospora crassa (1977), Biochem. J., 165, 11-18.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.59

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0095
-
 S-adenosyl-L-methionine
-
 Neurospora crassa
0.12
-
 [cytochrome c]-L-lysine
-
 Neurospora crassa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 74.3% of the enzyme activity Neurospora crassa 5829
-
lipid bilayer 13.6% of the enzyme activity Neurospora crassa
-
-
membrane
-
 Neurospora crassa 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytochrome c L-lysine Neurospora crassa
-
 S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Neurospora crassa

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + [cytochrome c]-L-lysine = S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine specific methylation site, 1 lysine in cytochrome c, comparison Neurospora crassa
a

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000289
-
 partially purified enzyme Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytochrome c L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
S-adenosyl-L-methionine + cytochrome c L-lysine does not randomly methylate cytochrome c, but shows absolute specificity for some amino acid sequences Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
S-adenosyl-L-methionine + myoglobin L-lysine 10% activity compared to cytochrome c Neurospora crassa S-adenosyl-L-homocysteine + myoglobin N6-methyl-L-lysine
-
 ?
S-adenosyl-L-methionine + [cytochrome c]-L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Neurospora crassa