Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nsp10 protein | nsp10 acts as an essential trigger to activate the 2'-O-methyltransferase activity of nsp16. The region of amino acids 42120 in nsp10 is needed for stimulating the nsp16 2'-O-methyltransferase activity | Severe acute respiratory syndrome-related coronavirus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Severe acute respiratory syndrome-related coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | peptides K12 (aa 68-80 of nsp10), and K29 (aa 68-96 of nsp10) decrease the S-adenosyl-L-methionine (SAM) binding by nsp16 in a dose-dependent manner. When 0.4 mM of K29 is used, it almost abolishes the SAM-binding activity of nsp16. In contrast, the peptides K8 (aa 100-107 of nsp10), K10 (aa 91-100 of nsp10) and K20 (aa 40-59 of nsp10), which cannot inhibit the 2'-O-MTase activity of nsp16, have no effect on SAM binding of nsp16, even when the final concentration of 0.5 mM is applied | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Severe acute respiratory syndrome-related coronavirus WHU | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + m7G(5')pppA-RNA | - |
Severe acute respiratory syndrome-related coronavirus | S-adenosyl-L-homocysteine + m7G(5')pppAm-RNA | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppA-RNA | - |
Severe acute respiratory syndrome-related coronavirus WHU | S-adenosyl-L-homocysteine + m7G(5')pppAm-RNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2'-O-methyltransferase | - |
Severe acute respiratory syndrome-related coronavirus |
2'-O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |
2'O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |
nsp10/nsp16 complex | - |
Severe acute respiratory syndrome-related coronavirus |
RNA cap 2'-O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |