Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Dengue virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | - |
Dengue virus | |
sinefungin | - |
Dengue virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
S-adenosyl-L-methionine | apparent value, in glycine buffer (pH 10.0), at 22°C | Dengue virus | |
1.8 | - |
m7G(5')pppG-RNA | apparent value, in glycine buffer (pH 10.0), at 22°C | Dengue virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GSTPrep 16/10 column chromatography | Dengue virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + m7G(5')pppG-RNA | m7GTP capped nucleotides 1-110. 2'-O MTase activity is seen only in glycine buffer, at pH 10, with a minimum RNA length of 20 nucleotides needed | Dengue virus | S-adenosyl-L-homocysteine + m7G(5')pppGm-RNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2'-O MTase | - |
Dengue virus |
2'O methyltransferase | - |
Dengue virus |
NS5 MTase | - |
Dengue virus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000041 | - |
in glycine buffer (pH 10.0), at 22°C | Dengue virus | sinefungin | |
0.00049 | - |
in glycine buffer (pH 10.0), at 22°C | Dengue virus | S-adenosyl-L-homocysteine |