Literature summary for 2.1.1.56 extracted from

Thillier, Y.; Decroly, E.; Morvan, F.; Canard, B.; Vasseur, J.J.; Debart, F.
Synthesis of 5' cap-0 and cap-1 RNAs using solid-phase chemistry coupled with enzymatic methylation by human (guanine-N7)-methyl transferase (2012), RNA, 18, 856-868.

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Application

Application	Comment	Organism
synthesis	combination of chemical synthesis and enzymatic methylation in order to produce large amounts of RNA oligonucleotides carrying a cap-0 or cap-1. Short RNAs are synthesized on solid support by the phosphoramidite 2'-O-pivaloyloxymethyl chemistry. The cap structure is then coupled by the addition of GDP after phosphorylation of the terminal 5'-OH and activation by imidazole. GpppN-RNAs or GpppN2'-Om-RNAs are purified before the N7-methyl group is added using the human (guanine-N7)-methyl transferase to yield 7mGpppN-RNAs (cap-0) or 7mGpppN29-Om-RNAs (cap-1)	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O43148	-	-

Synonyms

Synonyms	Comment	Organism
RNMT	-	Homo sapiens

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Release 2023.1 (January 2023)