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Literature summary for 2.1.1.56 extracted from

  • Chen, Y.; Cai, H.; Pan, J.; Xiang, N.; Tien, P.; Ahola, T.; Guo, D.
    Functional screen reveals SARS coronavirus nonstructural protein nsp14 as a novel cap N7 methyltransferase (2009), Proc. Natl. Acad. Sci. USA, 106, 3484-3489.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strains YBS40, YBS2, and YBS20 Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
D243A the point mutation in the ExoN domain does not significantly interfere with the N7-MTase function Severe acute respiratory syndrome-related coronavirus
D331A the mutation destroys the MTase activity of nsp14 in vivo Severe acute respiratory syndrome-related coronavirus
D90A/E92A the point mutation in the ExoN domain does not significantly interfere with the N7-MTase function Severe acute respiratory syndrome-related coronavirus
H268L the point mutation in the ExoN domain does not significantly interfere with the N7-MTase function Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA
-
Severe acute respiratory syndrome-related coronavirus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?

Synonyms

Synonyms Comment Organism
(guanine-N7)-methyltransferase
-
Severe acute respiratory syndrome-related coronavirus
N7-MTase
-
Severe acute respiratory syndrome-related coronavirus
nonstructural protein 14
-
Severe acute respiratory syndrome-related coronavirus
nsp 14
-
Severe acute respiratory syndrome-related coronavirus