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Literature summary for 2.1.1.56 extracted from

  • Schwer, B.; Shuman, S.
    Genetic analysis of poxvirus mRNA cap methyltransferase: suppression of conditional mutations in the stimulatory D12 subunit by second-site mutations in the catalytic D1 subunit (2006), Virology, 352, 145-156.
    View publication on PubMed
Search for more literature for 2.1.1.56

Application

Application Comment Organism
analysis enzyme is composed of catalytic vD1 (498–844) and stimulatory vD12 subunits, yeast is a powerful system for suppressor analysis of interacting poxvirus proteins, screen of amino acids in vD1-C, at which mutations restore methyltransferase function in conjunction with defective vD12 proteins, reference to the crystal structure of the microsporidian cap methyltransferase suggests that distinct functional classes of suppressors are selected Vaccinia virus

Cloned(Commentary)

Cloned (Comment) Organism
wild-type or mutant vD12, into the pRS413T vector, expressed in Saccharomyces cerevisiae strain YBS40 Vaccinia virus

Protein Variants

Protein Variants Comment Organism
C173A/S174A modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C Vaccinia virus
D192A/S193A temperature-sensitive vD12 allele, near-lethality, only grows very slowly at 19°C Vaccinia virus
D52A/L53A modest defect, grows well at 30°C, but slowly at 34°C and not at 37°C Vaccinia virus
E104A/G11A modest defect, grows well at 19, 30 and 34 °C, but slowly at 37°C Vaccinia virus
E24A-25A temperature-sensitive vD12 allele, does not grow at 37°C Vaccinia virus
E275A/N276A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
F141A/R142A grows slowly at 19 and 30°C and fails to grow at 34°C or higher Vaccinia virus
F141A/R142A temperature-sensitive vD12 allele, does not grow above 34°C Vaccinia virus
F176A/K177A lethal at all temperatures Vaccinia virus
F44A/L45A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
H260A/S261A modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C Vaccinia virus
I47A/S48A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
K111A/R112A modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C Vaccinia virus
K156A viable at 19°C and 30°C, but fails to grow at 34°C Vaccinia virus
K156A/L157A lethal at all temperatures Vaccinia virus
K223A/D223A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
L167A viable at 19°C and 30°C, but fails to grow at 34°C Vaccinia virus
L16A/P17A temperature-sensitive vD12 allele, forms small colonies at 19°C and fails to grow at 30°C or higher Vaccinia virus
N120A/N121A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
N126A/N127A modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C Vaccinia virus
N42A-Y43A temperature-sensitive vD12 allele, does not grow at 37°C Vaccinia virus
N54A/R55A modest defect, grows well at 19 and 30°C, but slowly at 34 and very slowly at 37°C Vaccinia virus
P158A/T159A temperature-sensitive vD12 allele, only grows at 19°C Vaccinia virus
P35A/S36A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
R280A-R281A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
S186A/D187A modest defect, grows well at 19, 30 and 34°C, but very slowly at 37°C Vaccinia virus
W189A/L190A temperature-sensitive vD12 allele, does not grow at 37°C Vaccinia virus
Y258A-V259A modest defect, grows well at 19, 30 and 34°C, but slowly at 37°C Vaccinia virus
Y39A/G40A temperature-sensitive vD12 allele, does not grow at 37°C Vaccinia virus

Organism

Organism UniProt Comment Textmining
Vaccinia virus
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Synonyms

Synonyms Comment Organism
guanine-N7 methyltransferase
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 Vaccinia virus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
19 37
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 Vaccinia virus