Application | Comment | Organism |
---|---|---|
analysis | second Trypanosoma brucei RNA (guanine N-7) cap methyltransferase, named TbCgm1, TbCmt1 is homologous with TbCgm1, consisting of a C-terminal (guanine N-7) methyltransferase domain and an N-terminal guanylyltransferase domain, which contains signature motifs found in the nucleotidyl transferase superfamily | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
TbCmt1 inserted into an inducible T7 RNA polymerase-based pET vector and introduced into Escherichia coli BL21(DE3)Gold | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
D144A | TbCmt1 mutant shows 5% of wild-type activity | Trypanosoma brucei |
D80A | TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity | Trypanosoma brucei |
E258A | TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity | Trypanosoma brucei |
F171A | TbCmt1 mutant displays near-wild-type activity | Trypanosoma brucei |
H174A | TbCmt1 mutant displays near-wild-type activity | Trypanosoma brucei |
K83A | TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity | Trypanosoma brucei |
V241A | TbCmt1 mutant displays near-wild-type activity | Trypanosoma brucei |
V242A | TbCmt1 mutant displays near-wild-type activity | Trypanosoma brucei |
Y175A | TbCmt1 mutant shows 3% of wild-type activity | Trypanosoma brucei |
Y246A | TbCmt1 mutant shows 2% of wild-type activity | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE of His-tagged TbCmt1 | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TbCmt1 protein purified from soluble extract by nickel-agarose, followed by phosphocellulose chromatography | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + G(5')pppN | TbCmt1 | Trypanosoma brucei | S-adenosyl-L-homocysteine + m7G(5')pppG | - |
? | |
S-adenosyl-L-methionine + GpppA | TbCmt1 | Trypanosoma brucei | S-adenosyl-L-homocysteine + m7GpppA | - |
? | |
S-adenosyl-L-methionine + GpppG | TbCmt1 | Trypanosoma brucei | S-adenosyl-L-homocysteine + m7GpppG | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE of His-tagged TbCmt1 | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
RNA cap (guanine N-7) methyltransferase | - |
Trypanosoma brucei |
TbCgm1 | - |
Trypanosoma brucei |
TbCmt1 | - |
Trypanosoma brucei |