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Literature summary for 2.1.1.56 extracted from

  • Guo, P.; Moss, B.
    Interaction and mutual stabilization of the two subunits of vaccinia virus mRNA capping enzyme coexpressed in Escherichia coli (1990), Proc. Natl. Acad. Sci. USA, 87, 4023-4027.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli Vaccinia virus
coexpression of genes D1 and D12 from 1 plasmid Vaccinia virus
vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21 Vaccinia virus

General Stability

General Stability Organism
small and large subunit of the mRNA capping enzyme are not stable and active when isolated, respectively Vaccinia virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
components of the mRNA capping enzyme Vaccinia virus
33000
-
small subunit of mRNA capping enzyme , gel filtration, SDS-PAGE Vaccinia virus
97000
-
large subunit of mRNA capping enzyme, gel filtration, SDS-PAGE Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus guanylyltransferase and RNA-triphosphatase are located on the large subunit S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
small and large subunit of mRNA capping enzyme , recombinant from E. coli Vaccinia virus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA
-
Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
S-adenosyl-L-methionine + G(5')pppR-RNA the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA guanylyltransferase and RNA-triphosphatase are located on the large subunit Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?