Cloned (Comment) | Organism |
---|---|
2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli | Vaccinia virus |
coexpression of genes D1 and D12 from 1 plasmid | Vaccinia virus |
vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21 | Vaccinia virus |
General Stability | Organism |
---|---|
small and large subunit of the mRNA capping enzyme are not stable and active when isolated, respectively | Vaccinia virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
components of the mRNA capping enzyme | Vaccinia virus |
33000 | - |
small subunit of mRNA capping enzyme , gel filtration, SDS-PAGE | Vaccinia virus |
97000 | - |
large subunit of mRNA capping enzyme, gel filtration, SDS-PAGE | Vaccinia virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + G(5')pppR-RNA | Vaccinia virus | the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | Vaccinia virus | guanylyltransferase and RNA-triphosphatase are located on the large subunit | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | Vaccinia virus | one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vaccinia virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
small and large subunit of mRNA capping enzyme , recombinant from E. coli | Vaccinia virus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] | RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit | Vaccinia virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + G(5')pppR-RNA | - |
Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap | ? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | guanylyltransferase and RNA-triphosphatase are located on the large subunit | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? | |
S-adenosyl-L-methionine + G(5')pppR-RNA | one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone | Vaccinia virus | S-adenosyl-L-homocysteine + m7G(5')pppR-RNA | - |
? |