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Literature summary for 2.1.1.56 extracted from

  • Germershausen, J.; Goodman, D.; Somberg, E.W.
    5 Cap methylation of homologous poly A(+) RNA by a RNA (guanine-7) methyltransferase from Neurospora crassa (1978), Biochem. Biophys. Res. Commun., 82, 871-878.
    View publication on PubMed
Search for more literature for 2.1.1.56

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA Neurospora crassa associated with mRNA-guanylyltransferase S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
 mutant met-
-

Purification (Commentary)

Purification (Comment) Organism
DNA-agarose affinity chromatography Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Neurospora crassa
-
mycelium
-
 Neurospora crassa
-

Storage Stability

Storage Stability Organism
-20°C, in 10% v/v glycerol, at least several months Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppN N: G Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
S-adenosyl-L-methionine + G(5')pppN N: A Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
S-adenosyl-L-methionine + G(5')pppR-RNA unmethylated mRNA of reovirus and poly A(+) RNA Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
S-adenosyl-L-methionine + G(5')pppR-RNA associated with mRNA-guanylyltransferase Neurospora crassa S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Neurospora crassa