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Literature summary for 2.1.1.56 extracted from

  • Higman, M.A.; Bourgeois, N.; Niles, E.G.
    The vaccinia virus mRNA (guanine-N7-)-methyltransferase requires both subunits of the mRNA capping enzyme for activity (1992), J. Biol. Chem., 267, 16430-16437.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21 Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus guanylyltransferase and RNA-triphosphatase are located on the large subunit S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
small and large subunit of mRNA capping enzyme , recombinant from E. coli Vaccinia virus

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + G(5')pppR-RNA
-
Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
S-adenosyl-L-methionine + G(5')pppR-RNA guanylyltransferase and RNA-triphosphatase are located on the large subunit Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?
S-adenosyl-L-methionine + G(5')pppR-RNA one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Vaccinia virus