Literature summary for 2.1.1.53 extracted from

Teuber, M.; Azemi, M.E.; Namjoyan, F.; Meier, A.C.; Wodak, A.; Brandt, W.; Draeger, B.
Putrescine N-methyltransferases-a structure-function analysis (2007), Plant Mol. Biol., 63, 787-801.

Search for more literature for 2.1.1.53

Application

Application	Comment	Organism
additional information	protein model of PMT based on the crystal structure of SPDS suggests that overall protein folds are comparable, the respective cosubstrates S-adenosylmethionine and decarboxylated S-adenosylmethionine, however, appear to bind differentially to the active sites of both enzymes, and the substrate putrescine adopts a different position	Datura stramonium

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Rosetta-gami (DE3) cells	Solanum lycopersicum
expressed in Rosetta-gami (DE3) cells	Calystegia sepium
expressed in Rosetta-gami (DE3) cells	Physalis divaricata
expressed in Rosetta-gami (DE3) cells	Solanum dulcamara
expressed in Rosetta-gami (DE3) cells	Datura inoxia
expressed in Rosetta-gami (DE3) cells	Datura stramonium
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells	Physalis divaricata
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells	Calystegia sepium
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells	Solanum dulcamara
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells	Datura inoxia
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells	Datura stramonium
into vector pET21d and transferred into Escherichia coli Rosetta-gami (DE3) cells	Solanum lycopersicum

Protein Variants

Protein Variants	Comment	Organism
I136V	reduced PMT activity, about half of the wild-type activity	Datura stramonium
I141D	no PMT activity	Datura stramonium
I141D	mutant shows no activity	Datura stramonium
T117Q	no PMT activity	Datura stramonium
T117Q	mutant shows no activity	Datura stramonium
T117Q/I141D	no PMT activity	Datura stramonium
T117Q/I141D	mutant shows no activity	Datura stramonium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.113	-	putrescine	-	Solanum lycopersicum
0.12	-	putrescine	PMT1	Calystegia sepium
0.12	-	putrescine	isoenzyme PMT1	Calystegia sepium
0.169	-	putrescine	-	Solanum dulcamara
0.281	-	putrescine	-	Datura stramonium

Organism

Organism	UniProt	Comment	Textmining
Calystegia sepium	-	-	-
Calystegia sepium	Q2KTH3	-	-
Datura inoxia	Q2KTH1	-	-
Datura inoxia	Q2KTH2	-	-
Datura stramonium	Q70EW6	-	-
Physalis divaricata	Q2KTH0	-	-
Solanum dulcamara	-	-	-
Solanum lycopersicum	-	-	-
Solanum lycopersicum	Q2KTH4	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration, approximately 95% pure	Physalis divaricata
gel filtration, approximately 95% pure	Calystegia sepium
gel filtration, approximately 95% pure	Solanum dulcamara
gel filtration, approximately 95% pure	Datura inoxia
gel filtration, approximately 95% pure	Datura stramonium
gel filtration, approximately 95% pure	Solanum lycopersicum

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Solanum lycopersicum	-
root	-	Calystegia sepium	-
root	-	Physalis divaricata	-
root	-	Solanum dulcamara	-
root	-	Datura inoxia	-
root	-	Datura stramonium	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + putrescine	-	Solanum lycopersicum	S-adenosyl-L-homocysteine + N-methylputrescine	-	?
S-adenosyl-L-methionine + putrescine	-	Calystegia sepium	S-adenosyl-L-homocysteine + N-methylputrescine	-	?
S-adenosyl-L-methionine + putrescine	-	Physalis divaricata	S-adenosyl-L-homocysteine + N-methylputrescine	-	?
S-adenosyl-L-methionine + putrescine	-	Solanum dulcamara	S-adenosyl-L-homocysteine + N-methylputrescine	-	?
S-adenosyl-L-methionine + putrescine	-	Datura inoxia	S-adenosyl-L-homocysteine + N-methylputrescine	-	?
S-adenosyl-L-methionine + putrescine	-	Datura stramonium	S-adenosyl-L-homocysteine + N-methylputrescine	-	?

Synonyms

Synonyms	Comment	Organism
PMT	-	Solanum lycopersicum
PMT	-	Physalis divaricata
PMT	-	Solanum dulcamara
PMT	-	Datura stramonium
PMT1	-	Calystegia sepium
PMT1	-	Datura inoxia
PMT2	-	Calystegia sepium
PMT2	-	Datura inoxia
putrescine N-methyltransferase	-	Solanum lycopersicum
putrescine N-methyltransferase	-	Calystegia sepium
putrescine N-methyltransferase	-	Physalis divaricata
putrescine N-methyltransferase	-	Solanum dulcamara
putrescine N-methyltransferase	-	Datura inoxia
putrescine N-methyltransferase	-	Datura stramonium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Solanum lycopersicum
30	-	assay at	Calystegia sepium
30	-	assay at	Physalis divaricata
30	-	assay at	Solanum dulcamara
30	-	assay at	Datura inoxia
30	-	assay at	Datura stramonium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.047	-	putrescine	-	Solanum dulcamara
0.086	-	putrescine	PMT2	Calystegia sepium
0.086	-	putrescine	isoenzyme PMT2	Calystegia sepium
0.16	-	putrescine	PMT1	Calystegia sepium
0.16	-	putrescine	isoenzyme PMT1	Calystegia sepium
0.19	-	putrescine	-	Solanum lycopersicum
0.198	-	putrescine	-	Physalis divaricata
0.359	-	putrescine	-	Datura inoxia
0.383	-	putrescine	-	Datura inoxia
0.383	-	putrescine	PMT1	Datura inoxia
0.388	-	putrescine	-	Datura stramonium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Physalis divaricata
9	-	-	Calystegia sepium
9	-	-	Solanum dulcamara
9	-	-	Datura inoxia
9	-	-	Datura stramonium
9	-	-	Solanum lycopersicum
9	-	assay at	Solanum lycopersicum
9	-	assay at	Calystegia sepium
9	-	assay at	Physalis divaricata
9	-	assay at	Solanum dulcamara
9	-	assay at	Datura inoxia
9	-	assay at	Datura stramonium

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Release 2023.1 (January 2023)