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Literature summary for 2.1.1.5 extracted from

  • Zhang, B.; Denomme, M.M.; White, C.R.; Leung, K.Y.; Lee, M.B.; Greene, N.D.; Mann, M.R.; Trasler, J.M.; Baltz, J.M.
    Both the folate cycle and betaine-homocysteine methyltransferase contribute methyl groups for DNA methylation in mouse blastocysts (2015), FASEB J., 29, 1069-1079.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Mus musculus
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Source Tissue

Source Tissue Comment Organism Textmining
blastocyst
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Mus musculus
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General Information

General Information Comment Organism
physiological function in blastocysts, mean inner cell numbers decrease from18-19 in controls to 11-13 when the folate cycle is inhibited by the antifolate methotrexate and to 12-14 when BHMT expression is knocked down by antisense morpholinos. Inhibiting both pathways more severely affects inner cellmass development (7-8 cells).Total SAM levels in mouse blastocysts decrease significantly only when both pathways are inhibited (from 3.1 to 1.6 pmol/100 blastocysts). DNA methylation is minimally affected by inhibition of either pathway alone but decreases by at least 45-55% when both BHMT and the folate cycle are inhibited simultaneously Mus musculus