Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-cysteine | increase of product formation | Rattus norvegicus | |
dithiothreitol | - |
Rattus norvegicus | |
L-cysteine | stimulation at low concentration | Rattus norvegicus | |
additional information | taurine, cysteic acid, cysteine sulfinate, sulfate, glycine, L-serine, L-threonine do not affect activity | Rattus norvegicus | |
S-[(2R)-2-amino-2-carboxyethyl]-L-homocysteine | - |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Cystine | - |
Rattus norvegicus | |
L-cysteine | - |
Rattus norvegicus | |
L-cystine | - |
Rattus norvegicus | |
additional information | not inhibitory: taurine, cysteic acid, cysteine sulfinate, sulfate, glycine, L-serine, L-threonine; product inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | 0.021 | L-homocysteine | - |
Rattus norvegicus | |
0.039 | 0.056 | betaine | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N,N-trimethylglycine + L-homocysteine | Rattus norvegicus | i.e. betaine, involved in betaine metabolism | N,N-dimethylglycine + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
betaine + L-homocysteine = dimethylglycine + L-methionine | ordered bi-bi mechanism with homocysteine the first substrate to be added and methionine the last product released | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N,N-trimethylglycine + L-homocysteine | - |
Rattus norvegicus | N,N-dimethylglycine + L-methionine | - |
? | |
N,N,N-trimethylglycine + L-homocysteine | i.e. betaine, involved in betaine metabolism | Rattus norvegicus | N,N-dimethylglycine + L-methionine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
Tris, TES and HEPES decrease activity | Rattus norvegicus |
8 | - |
0.05 M Tricine buffer | Rattus norvegicus |