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Literature summary for 2.1.1.5 extracted from
- Betaine-homocysteine S-methyltransferase (human) (1987), Methods Enzymol., 143, 384-388.
General Stability
| General Stability | Organism |
|---|---|
| divalent cations and 2-mercaptoethanol do not stabilize during purification | Homo sapiens |
| lyophilization inactivates | Homo sapiens |
| substrates stabilize during purification | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3-Dimethylbutyrate | competitive to betaine | Homo sapiens |  |
| Butyrate | moderate, competitive to betaine | Homo sapiens | |
| Isovalerate | competitive to betaine | Homo sapiens | |
| additional information | no inhibition by sarcosine | Homo sapiens | |
| S-(delta-Carboxybutyl)-DL-homocysteine | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
6 * 45000, SDS-PAGE | Homo sapiens |
| 270000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
- |
- |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two forms of the enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
| kidney | - |
Equus caballus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Equus caballus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.314 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, over a year in 50% v/v glycerol | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N,N-trimethylglycine + L-homocysteine | - |
Homo sapiens | N,N-dimethylglycine + L-methionine | - |
? | |
| N,N,N-trimethylglycine + L-homocysteine | - |
Equus caballus | N,N-dimethylglycine + L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 45000, SDS-PAGE | Homo sapiens |
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