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Literature summary for 2.1.1.49 extracted from

  • Ansher, S.S.; Jakoby, W.B.
    Amine N-methyltransferases from rabbit liver (1986), J. Biol. Chem., 261, 3996-4001.
    View publication on PubMed
Search for more literature for 2.1.1.49

Inhibitors

Inhibitors Comment Organism Structure
additional information substrate inhibition, but not by tryptamine and 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline Oryctolagus cuniculus
N,N-Dimethyltryptamine competitive inhibitor Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline transferase A, pH 7.6 Oryctolagus cuniculus
0.015
-
 S-adenosyl-L-methionine 1 mM tryptamine as methyl acceptor, pH 8.5 Oryctolagus cuniculus
0.12
-
 tryptamine transferase B, pH 8.5 Oryctolagus cuniculus
0.2
-
 imidazole transferase A, pH 8.5 Oryctolagus cuniculus
0.24
-
 desmethylimipramine transferase B, pH 8.5 Oryctolagus cuniculus
0.3
-
 tryptamine transferase A, pH 8.5 Oryctolagus cuniculus
0.3
-
 desmethylimipramine transferase A, pH 8.5 Oryctolagus cuniculus
1.5
-
 pyrazole transferase A, pH 7.6 Oryctolagus cuniculus
2
-
 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline transferase B, pH 7.6 Oryctolagus cuniculus
3.2
-
 pyrazole transferase B, pH 7.6 Oryctolagus cuniculus
5.1
-
 imidazole transferase B, pH 8.5 Oryctolagus cuniculus
7.6
-
 aniline transferase A, pH 7.6 Oryctolagus cuniculus
7.8
-
 aniline transferase B, pH 7.6 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition of transferase A Oryctolagus cuniculus
30000
-
 gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + an amine Oryctolagus cuniculus enzymes participate in detoxication, recycling of drugs after initial demethylation S-adenosyl-L-homocysteine + an N-methylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 amine N-methyltransferase A and B
-
Oryctolagus cuniculus
-
 10-16 week old
-
Oryctolagus cuniculus New Zealand
-
 10-16 week old
-

Purification (Commentary)

Purification (Comment) Organism
two N-methyltransferases A and B separable by ammonium sulfate fractionation, HPLC-ion exchange chromatography Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver two amine N-methyltransferases, A and B Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000068
-
 N-methyltransferase A, aniline Oryctolagus cuniculus
0.00022
-
 N-methyltransferase B, desmethylimipramine Oryctolagus cuniculus
0.00026
-
 N-methyltransferase A, desmethylimipramine Oryctolagus cuniculus
0.00044
-
 N-methyltransferase A, tryptamine Oryctolagus cuniculus
0.00127
-
 N-methyltransferase B, aniline Oryctolagus cuniculus
0.0031
-
 N-methyltransferase B, tryptamine Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-80°C, partially purified, per month, less than 10% loss of activity Oryctolagus cuniculus
-80°C, purified, per month, about 25% loss of activity Oryctolagus cuniculus
2°C, per week, 25% loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information two N-methyltransferases A and B with very broad but overlapping substrate specificity for primary and secondary amines, lipophilic substrates, overview poor substrates, no substrates: N-acetylserotonine, 4-aminoacetophenone, 4-aminophenol, benzamide, 4-chloroaniline, deoxyepinephrine, 1,4-diaminobutane, dopamine, 1-ethanol-2-amine, ethylenediamine, histamine, histidine methylester, 3-hydroxytryptamine, indole, pyrrole, methylamine, N-methyldopamine, nicotinamide, octopamine, phenylalanine, phenylethanolamine, theophylline, tryptophan, tyrosine, N,N-dimethyltryptamine Oryctolagus cuniculus ?
-
 ?
additional information two N-methyltransferases A and B with very broad but overlapping substrate specificity for primary and secondary amines, lipophilic substrates, overview poor substrates, no substrates: N-acetylserotonine, 4-aminoacetophenone, 4-aminophenol, benzamide, 4-chloroaniline, deoxyepinephrine, 1,4-diaminobutane, dopamine, 1-ethanol-2-amine, ethylenediamine, histamine, histidine methylester, 3-hydroxytryptamine, indole, pyrrole, methylamine, N-methyldopamine, nicotinamide, octopamine, phenylalanine, phenylethanolamine, theophylline, tryptophan, tyrosine, N,N-dimethyltryptamine Oryctolagus cuniculus New Zealand ?
-
 ?
S-adenosyl-L-methionine + 5-methyloxytryptamine higher activity with transferase A Oryctolagus cuniculus ?
-
 ?
S-adenosyl-L-methionine + 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline substrate with highest apparent kcat Oryctolagus cuniculus ?
-
 ?
S-adenosyl-L-methionine + alpha-N-methyltryptamine
-
 Oryctolagus cuniculus S-adenosyl-L-homocysteine + alpha,alpha-N,N-dimethyltryptamine
-
 ?
S-adenosyl-L-methionine + alpha-N-methyltryptamine
-
 Oryctolagus cuniculus New Zealand S-adenosyl-L-homocysteine + alpha,alpha-N,N-dimethyltryptamine
-
 ?
S-adenosyl-L-methionine + an amine enzymes participate in detoxication, recycling of drugs after initial demethylation Oryctolagus cuniculus S-adenosyl-L-homocysteine + an N-methylamine
-
 ?
S-adenosyl-L-methionine + aniline poor substrate Oryctolagus cuniculus S-adenosyl-L-homocysteine + N-methylaniline
-
 ?
S-adenosyl-L-methionine + anisidine higher activity with transferase B Oryctolagus cuniculus ?
-
 ?
S-adenosyl-L-methionine + desmethylimipramine higher activity with transferase A Oryctolagus cuniculus S-adenosyl-L-homocysteine + imipramine
-
 ?
S-adenosyl-L-methionine + desmethylimipramine higher activity with transferase A Oryctolagus cuniculus New Zealand S-adenosyl-L-homocysteine + imipramine
-
 ?
S-adenosyl-L-methionine + imidazole higher activity with transferase A Oryctolagus cuniculus ?
-
 ?
S-adenosyl-L-methionine + N-methylbutylamine only transferase A Oryctolagus cuniculus S-adenosyl-L-homocysteine + N,N-dimethylbutylamine
-
 ?
S-adenosyl-L-methionine + N-methylcyclohexylamine only transferase A Oryctolagus cuniculus S-adenosyl-L-homocysteine + N,N-dimethylcyclohexylamine
-
 ?
S-adenosyl-L-methionine + N-methyltryptamine
-
 Oryctolagus cuniculus S-adenosyl-L-homocysteine + N,N-dimethyltryptamine
-
 ?
S-adenosyl-L-methionine + N-methyltryptamine
-
 Oryctolagus cuniculus New Zealand S-adenosyl-L-homocysteine + N,N-dimethyltryptamine
-
 ?
S-adenosyl-L-methionine + phenyltetrahydropyridine higher activity with transferase A Oryctolagus cuniculus ?
-
 ?
S-adenosyl-L-methionine + pyrazole higher activity with transferase A Oryctolagus cuniculus ?
-
 ?
S-adenosyl-L-methionine + tryptamine i.e. 3-[2-aminoethyl] indole, best substrate, two N-methyltransferases: A and B, non-specific Oryctolagus cuniculus S-adenosyl-L-homocysteine + alpha-N-methyltryptamine
-
 ?
S-adenosyl-L-methionine + tryptamine i.e. 3-[2-aminoethyl] indole, best substrate, two N-methyltransferases: A and B, non-specific Oryctolagus cuniculus New Zealand S-adenosyl-L-homocysteine + alpha-N-methyltryptamine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Oryctolagus cuniculus
More aggregation upon denaturation leads to 60 kDa and 90 kDa fractions Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0333
-
 desmethylimipramine transferase B, pH 8.5 Oryctolagus cuniculus
0.0333
-
 aniline transferase A, pH 7.6 Oryctolagus cuniculus
0.0833
-
 imidazole transferase A, pH 8.5 Oryctolagus cuniculus
0.1
-
 pyrazole transferase B, pH 7.6 Oryctolagus cuniculus
0.117
-
 S-adenosyl-L-methionine transferase B, pH 8.5 Oryctolagus cuniculus
0.167
-
 imidazole transferase B, pH 8.5 Oryctolagus cuniculus
0.2
-
 S-adenosyl-L-methionine transferase A, pH 8.5 Oryctolagus cuniculus
0.233
-
 desmethylimipramine transferase A, pH 8.5 Oryctolagus cuniculus
0.25
-
 pyrazole transferase A, pH 7.6 Oryctolagus cuniculus
0.367
-
 aniline transferase B, pH 7.6 Oryctolagus cuniculus
0.4
-
 tryptamine transferase A, pH 8.5 Oryctolagus cuniculus
0.45
-
 tryptamine transferase B, pH 8.5 Oryctolagus cuniculus
0.667
-
 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline transferase A, pH 7.6 Oryctolagus cuniculus
3.67
-
 7,8-dichloro-1,2,3,4-tetrahydroisoquinoline transferase B, pH 7.6 Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 transferase A: pI 4.9, transferase B: pI 5.1 Oryctolagus cuniculus
8.5
-
-
 Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8.9 tryptamine, about half-maximal activity at pH 7.2 and 8.9, transferase A Oryctolagus cuniculus
7.5 8.8 tryptamine, about half-maximal activity at pH 7.5 and 90% at pH 8.8, transferase B Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.2
-
 optimal for transferase A Oryctolagus cuniculus
7.8
-
 optimal for transferase B Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.006
-
 N,N-Dimethyltryptamine N-methyltransferase B Oryctolagus cuniculus
0.01
-
 N,N-Dimethyltryptamine N-methyltransferase A Oryctolagus cuniculus