Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells | Plasmodium falciparum |
expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells | Plasmodium vivax |
Protein Variants | Comment | Organism |
---|---|---|
C490A | inactive | Plasmodium falciparum |
C506A | inactive | Plasmodium vivax |
C506D | inactive | Plasmodium vivax |
C506F | inactive | Plasmodium vivax |
C506P | inactive | Plasmodium vivax |
C506Y | inactive | Plasmodium vivax |
R470D | inactive | Plasmodium falciparum |
R486D | inactive | Plasmodium vivax |
R486L | inactive | Plasmodium vivax |
R486Y | inactive | Plasmodium vivax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Plasmodium vivax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
methotrexate-Sepharose column chromatography and anion exchange column chromatography | Plasmodium falciparum |
methotrexate-Sepharose column chromatography and anion exchange column chromatography | Plasmodium vivax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium falciparum | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Plasmodium vivax | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Plasmodium falciparum |
homodimer | - |
Plasmodium vivax |