Literature summary for 2.1.1.45 extracted from

Pena, M.M.; Melo, S.P.; Xing, Y.Y.; White, K.; Barbour, K.W.; Berger, F.G.
The intrinsically disordered N-terminal domain of thymidylate synthase targets the enzyme to the ubiquitin-independent proteasomal degradation pathway (2009), J. Biol. Chem., 284, 31597-31607.

Search for more literature for 2.1.1.45

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in RJK88.13 cells and in HEp2/500 cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
G5L/S6A	the mutant has a relatively short half-life of about 1-2 h	Mus musculus
P2A	the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h	Mus musculus
P2D	the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h	Mus musculus
P2G	the mutant is unstable relative to wild type enzyme having half-life of 6-8 h similar to wild type	Mus musculus
P2R	the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h	Mus musculus
P2V	the mutant is unstable relative to wild type enzyme having half-life of 1-2 h with half-life of more than 36-48 h	Mus musculus
P2W	the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h	Mus musculus
P2Y	the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
acetylation	Nalpha-acetylation inhibits thymidylate degradation, Pro-2 protects the N terminus against Nalpha-acetylation	Mus musculus

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Blue-Sepharose column chromatography and HiTrap Q column chromatography	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + dUMP	-	Mus musculus	dihydrofolate + dTMP	-	?

Cofactor

Cofactor	Comment	Organism	Structure
N5,N10-methylenetetrahydrofolate	-	Mus musculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)