Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
in complexes with the dihydrotriazine-based or quinazoline-based antifolates C-448, cycloguanil, and Q-8, microbatch method, using 0.1-0.2 M ammonium acetate, 20-22% (w/v) PEG 4000 and 0.1 M sodium citrate buffer pH 5.6 | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58800 | - |
calculated from amino acid sequence | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
methotrexate affinity column chromatography | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma cruzi | dihydrofolate + dTMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHFR-TS | - |
Trypanosoma cruzi |
dihydrofolate reductase-thymidylate synthase | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
N5,N10-methylenetetrahydrofolate | - |
Trypanosoma cruzi |