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Literature summary for 2.1.1.45 extracted from
- Characteristics of NADPH-dependent thymidylate synthetase purified from Streptomyces aureofaciens (1992), Arch. Biochem. Biophys., 296, 81-87.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| beta-mercaptoethanol | - |
Mus musculus |  |
| beta-mercaptoethanol | essential for maximum activity | Kitasatospora aureofaciens | |
| dithioerythritol | - |
Kitasatospora aureofaciens | |
| dithiothreitol | - |
Mus musculus | |
| dithiothreitol | - |
Escherichia coli | |
| dithiothreitol | - |
Kitasatospora aureofaciens | |
General Stability
| General Stability | Organism |
|---|---|
| glycerol and Triton X-100 stabilize the enzyme | Kitasatospora aureofaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-fluoro-2'-deoxyuridylic acid | - |
Escherichia coli | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Kitasatospora aureofaciens | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Mus musculus | |
| 5-fluoro-2'-deoxyuridylic acid | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0033 | - |
2'-deoxyuridylate | - |
Crithidia fasciculata | |
| 0.00378 | - |
2'-deoxyuridylate | - |
Kitasatospora aureofaciens | |
| 0.0211 | - |
N5,N10-methylenetetrahydrofolate | - |
Kitasatospora aureofaciens | |
| 0.0253 | - |
methylenetetrahydrofolate | - |
Mus musculus | |
| 0.0389 | - |
NADPH | - |
Kitasatospora aureofaciens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | stimulates activity | Kitasatospora aureofaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
2 * 28000, SDS-PAGE | Kitasatospora aureofaciens |
| 77000 | - |
gel filtration | Kitasatospora aureofaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Saccharomyces cerevisiae | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Bos taurus | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Crithidia fasciculata | - |
dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | Kitasatospora aureofaciens | - |
dihydrofolate + dTMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Crithidia fasciculata | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Kitasatospora aureofaciens | - |
industrial strain BMK | - |
| Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
| Saccharomyces cerevisiae | - |
bakers' yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Kitasatospora aureofaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00652 | - |
- |
Kitasatospora aureofaciens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, TS activity of crude enzyme remains unchanged for 1 month of storage, purified enzyme loses 50% of its activity within 3-4 weeks | Kitasatospora aureofaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Saccharomyces cerevisiae | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Bos taurus | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Crithidia fasciculata | dihydrofolate + dTMP | - |
r | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Kitasatospora aureofaciens | dihydrofolate + dTMP | - |
? | |
| 5,10-methylenetetrahydrofolate + dUMP | - |
Kitasatospora aureofaciens | dihydrofolate + dTMP | - |
r | |
| additional information | - |
Escherichia coli | ? | - |
? | |
| additional information | monofunctional enzyme | Kitasatospora aureofaciens | ? | - |
? | |
| [6R,S]-5,10-methylenetetrahydropteroylglutamate + 2'-deoxyuridylate | - |
Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mus musculus |
| dimer | - |
Escherichia coli |
| dimer | - |
Saccharomyces cerevisiae |
| dimer | - |
Bos taurus |
| dimer | 2 * 28000, SDS-PAGE | Kitasatospora aureofaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Crithidia fasciculata |
| 30 | - |
- |
Kitasatospora aureofaciens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 60 | - |
Kitasatospora aureofaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Mus musculus |
| 7 | - |
- |
Escherichia coli |
| 7 | - |
- |
Saccharomyces cerevisiae |
| 7 | - |
- |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | absolute requirement | Kitasatospora aureofaciens | |
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