Inhibitors | Comment | Organism | Structure |
---|---|---|---|
10-formyl-5,8-dideazafolate | - |
Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
2 * 38500, SDS-PAGE | Mus musculus |
67000 | - |
- |
Lacticaseibacillus casei |
67000 | - |
Ehrlich ascites carcinoma cells, gel filtration | Mus musculus |
71000 | - |
- |
Lacticaseibacillus casei |
71000 | - |
Ehrlich ascites carcinoma cells, SDS-PAGE | Mus musculus |
75000 | - |
L1210 cells, gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Mus musculus | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Homo sapiens | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
HeLa cells | - |
Lacticaseibacillus casei | - |
- |
- |
Mus musculus | - |
L1210 cells | - |
Mus musculus | - |
Ehrlich ascites carcinoma cells | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
L1210 cells | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, after 5 months less than 25% activity is lost | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Mus musculus | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Homo sapiens | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | - |
Homo sapiens |
dimer | - |
Lacticaseibacillus casei |
dimer | 2 * 38500, SDS-PAGE | Mus musculus |