Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.42 extracted from

  • Lee, Y.; Kim, B.; Chong, Y.; Lim, Y.; Ahn, J.
    Cation dependent O-methyltransferases from rice (2008), Planta, 227, 641-647.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
OMT-15 and OMT-17 are expressed in Escherichia coli BL21(DE3) cells as glutathione S-transferase fusion proteins Oryza sativa

Protein Variants

Protein Variants Comment Organism
D168L the mutation abolishes the enzyme activity of OMT-15 Oryza sativa
D194L the mutation abolishes the enzyme activity of OMT-15 Oryza sativa
D209L the mutation abolishes the enzyme activity of OMT-17 Oryza sativa
D234L the mutation abolishes the enzyme activity of OMT-17 Oryza sativa
E69L the mutation results in about 14% loss of enzyme activity in OMT-15 and 40% loss of activity in OMT-17 Oryza sativa
N195I the mutation abolishes the enzyme activity of OMT-15 Oryza sativa
N235I the mutation abolishes the enzyme activity of OMT-17 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
luteolin OMT-15, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C Oryza sativa
0.104
-
luteolin OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Co2+ results in recovery of 82% of OMT-15 and 41% of OMT-17 activity compared to the addition of Mg2+ Oryza sativa
Mg2+ dependent on Oryza sativa
Mn2+ the addition of Mn2+ restores 74% of OMT-15 activity and 69% of OMT-17 compared to the addition of Mg2+ Oryza sativa
Zn2+ Zn2+ results in recovery of less than 10% of OMT-15 and OMT-17 activity compared to the addition of Mg2+ Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27800
-
OMT-15, calculated from amino acid sequence Oryza sativa
30600
-
recombinant OMT-17, SDS-PAGE Oryza sativa
31100
-
recombinant OMT-15, SDS-PAGE Oryza sativa
32000
-
OMT-17, calculated from amino acid sequence Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose 4 column chromatography Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
root OMT-15 is expressed higher in stem and roots than in other tissues Oryza sativa
-
stem OMT-17 is expressed in stems only, OMT-15 is expressed higher in stem and roots than in other tissues Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
luteolin + S-adenosyl-L-methionine OMT-15 shows 92% activity and OMT-17 shows 84% activity compared to myricetin Oryza sativa 3'-O-methylluteolin + S-adenosyl-L-homocysteine
-
?
additional information OMT-15 and -17 cannot utilize naringenin, apigenin, or kaempferol as substrates, taxifolin and eriodictyol do not serve as methyl acceptors Oryza sativa ?
-
?
S-adenosyl-L-methionine + 5-hydroxyferulic acid OMT-15 shows 25% activity and OMT-17 shows 18% activity compared to myricetin Oryza sativa ?
-
?
S-adenosyl-L-methionine + 7,8-dihydroxyflavone OMT-15 shows 102% activity and OMT-17 shows 143% activity compared to myricetin Oryza sativa ?
-
?

Synonyms

Synonyms Comment Organism
O-methyltransferase
-
Oryza sativa
OMT-15
-
Oryza sativa
OMT-17
-
Oryza sativa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000534
-
luteolin OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C Oryza sativa
0.00276
-
luteolin OMT-15, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C Oryza sativa