Cloned (Comment) | Organism |
---|---|
OMT-15 and OMT-17 are expressed in Escherichia coli BL21(DE3) cells as glutathione S-transferase fusion proteins | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
D168L | the mutation abolishes the enzyme activity of OMT-15 | Oryza sativa |
D194L | the mutation abolishes the enzyme activity of OMT-15 | Oryza sativa |
D209L | the mutation abolishes the enzyme activity of OMT-17 | Oryza sativa |
D234L | the mutation abolishes the enzyme activity of OMT-17 | Oryza sativa |
E69L | the mutation results in about 14% loss of enzyme activity in OMT-15 and 40% loss of activity in OMT-17 | Oryza sativa |
N195I | the mutation abolishes the enzyme activity of OMT-15 | Oryza sativa |
N235I | the mutation abolishes the enzyme activity of OMT-17 | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
luteolin | OMT-15, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C | Oryza sativa | |
0.104 | - |
luteolin | OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ results in recovery of 82% of OMT-15 and 41% of OMT-17 activity compared to the addition of Mg2+ | Oryza sativa | |
Mg2+ | dependent on | Oryza sativa | |
Mn2+ | the addition of Mn2+ restores 74% of OMT-15 activity and 69% of OMT-17 compared to the addition of Mg2+ | Oryza sativa | |
Zn2+ | Zn2+ results in recovery of less than 10% of OMT-15 and OMT-17 activity compared to the addition of Mg2+ | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27800 | - |
OMT-15, calculated from amino acid sequence | Oryza sativa |
30600 | - |
recombinant OMT-17, SDS-PAGE | Oryza sativa |
31100 | - |
recombinant OMT-15, SDS-PAGE | Oryza sativa |
32000 | - |
OMT-17, calculated from amino acid sequence | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose 4 column chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | OMT-15 is expressed higher in stem and roots than in other tissues | Oryza sativa | - |
stem | OMT-17 is expressed in stems only, OMT-15 is expressed higher in stem and roots than in other tissues | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
luteolin + S-adenosyl-L-methionine | OMT-15 shows 92% activity and OMT-17 shows 84% activity compared to myricetin | Oryza sativa | 3'-O-methylluteolin + S-adenosyl-L-homocysteine | - |
? | |
additional information | OMT-15 and -17 cannot utilize naringenin, apigenin, or kaempferol as substrates, taxifolin and eriodictyol do not serve as methyl acceptors | Oryza sativa | ? | - |
? | |
S-adenosyl-L-methionine + 5-hydroxyferulic acid | OMT-15 shows 25% activity and OMT-17 shows 18% activity compared to myricetin | Oryza sativa | ? | - |
? | |
S-adenosyl-L-methionine + 7,8-dihydroxyflavone | OMT-15 shows 102% activity and OMT-17 shows 143% activity compared to myricetin | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-methyltransferase | - |
Oryza sativa |
OMT-15 | - |
Oryza sativa |
OMT-17 | - |
Oryza sativa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000534 | - |
luteolin | OMT-17, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C | Oryza sativa | |
0.00276 | - |
luteolin | OMT-15, in 10 mM Tris-HCl buffer (pH 7.5), at 37°C | Oryza sativa |