Literature summary for 2.1.1.41 extracted from

Pereira, M.; Song, Z.; Santos-Silva, L.K.; Richards, M.H.; Nguyen, T.T.; Liu, J.; de Almeida Soares, C.M.; da Silva Cruz, A.H.; Ganapathy, K.; Nes, W.D.
Cloning, mechanistic and functional analysis of a fungal sterol C24-methyltransferase implicated in brassicasterol biosynthesis (2010), Biochim. Biophys. Acta, 1801, 1163-1174.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a recombinant fusion protein in Escherichia coli BL21	Paracoccidioides brasiliensis

Inhibitors

Inhibitors	Comment	Organism	Structure
25-azalanosterol	-	Paracoccidioides brasiliensis
26,27-dehydrolanosterol	-	Paracoccidioides brasiliensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.019	-	26,27-dehydrolanosterol	pH 7.5, 37°C, Vmax: 6 pmol/min	Paracoccidioides brasiliensis
0.03	-	Zymosterol	pH 7.5, 37°C, Vmax 2 pmol/min	Paracoccidioides brasiliensis
0.035	-	31-norlanosterol	pH 7.5, 37°C, Vmax 45 pmol/min	Paracoccidioides brasiliensis
0.038	-	lanosterol	pH 7.5, 37°C, Vmax: 50 pmol/min	Paracoccidioides brasiliensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42500	-	calculated from cDNA	Paracoccidioides brasiliensis

Organism

Organism	UniProt	Comment	Textmining
Paracoccidioides brasiliensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Paracoccidioides brasiliensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + 26,27-dehydrolanosterol	-	Paracoccidioides brasiliensis	?	-	?
S-adenosyl-L-methionine + 31-norlanosterol	-	Paracoccidioides brasiliensis	?	-	?
S-adenosyl-L-methionine + cycloartenol	-	Paracoccidioides brasiliensis	S-adenosyl-L-homocysteine + 24-methylenecycloartanol	-	?
S-adenosyl-L-methionine + lanosterol	-	Paracoccidioides brasiliensis	S-adenosyl-L-homocysteine + 24-methylene-24,25-dihydrolanosterol	-	?
S-adenosyl-L-methionine + zymosterol	-	Paracoccidioides brasiliensis	S-adenosyl-L-homocysteine + fecosterol	-	?

Synonyms

Synonyms	Comment	Organism
24-SMT	-	Paracoccidioides brasiliensis
sterol C24-methyltransferase	-	Paracoccidioides brasiliensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Paracoccidioides brasiliensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0023	-	lanosterol	pH 7.5, 37°C	Paracoccidioides brasiliensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Paracoccidioides brasiliensis

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	-	half maximum velocities at 6.5 and 9.0	Paracoccidioides brasiliensis
7.5	8	optimum pH	Paracoccidioides brasiliensis
9	-	half maximum velocities at 6.5 and 9.0	Paracoccidioides brasiliensis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000014	-	25-azalanosterol	pH 7.5, 37°C	Paracoccidioides brasiliensis
0.000054	-	26,27-dehydrolanosterol	pH 7.5, 37°C	Paracoccidioides brasiliensis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000014	-	pH 7.5, 37°C	Paracoccidioides brasiliensis	25-azalanosterol

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Release 2023.1 (January 2023)