Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
Y177F | mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type | Trypanosoma brucei |
Y208F | mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type | Trypanosoma brucei |
Y66F | mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type. Sterol composition of the Y66F catalysis is different. The sterol mixture possesses a significant decrease in ergosta-8,24(25)-dienol compensated by an increase in ergosta-8,25(27)-dienol and ergosta-8,24(28)-dienol | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
Zymosterol | mutant Y66F, pH not specified in the publication, 35°C | Trypanosoma brucei | |
0.01 | - |
Zymosterol | mutant Y177F, pH not specified in the publication, 35°C | Trypanosoma brucei | |
0.036 | - |
Zymosterol | mutant Y208F, pH not specified in the publication, 35°C | Trypanosoma brucei | |
0.055 | - |
Zymosterol | wild-type, pH not specified in the publication, 35°C | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + zymosterol | GC-MS analysis of the enzyme-generated products from a soluble TbSMT shows the presence of four biomethylated sterols identified as ergosta-8,25(27)-dienol, ergosta-8,24(28)-dienol, ergosta-8,24(25)-dienol and 24-dimethyl ergosta-8,25(27)-dienol in a ratio of substrate/product of 7:3. The products distributed in a ratio of approximately 5:1:2:2 respectively | Trypanosoma brucei | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sterol C24-methyltransferase | - |
Trypanosoma brucei |
TbSMT | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Trypanosoma brucei |