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Literature summary for 2.1.1.41 extracted from

  • Liu, J.; Ganapathy, K.; Wywial, E.; Bujnicki, J.M.; Nwogwugwu, C.A.; Nes, W.D.
    Effect of substrate features and mutagenesis of active site tyrosine residues on the reaction course catalysed by Trypanosoma brucei sterol C-24-methyltransferase (2011), Biochem. J., 439, 413-422.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
Y177F mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type Trypanosoma brucei
Y208F mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type Trypanosoma brucei
Y66F mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type. Sterol composition of the Y66F catalysis is different. The sterol mixture possesses a significant decrease in ergosta-8,24(25)-dienol compensated by an increase in ergosta-8,25(27)-dienol and ergosta-8,24(28)-dienol Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
Zymosterol mutant Y66F, pH not specified in the publication, 35°C Trypanosoma brucei
0.01
-
Zymosterol mutant Y177F, pH not specified in the publication, 35°C Trypanosoma brucei
0.036
-
Zymosterol mutant Y208F, pH not specified in the publication, 35°C Trypanosoma brucei
0.055
-
Zymosterol wild-type, pH not specified in the publication, 35°C Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + zymosterol GC-MS analysis of the enzyme-generated products from a soluble TbSMT shows the presence of four biomethylated sterols identified as ergosta-8,25(27)-dienol, ergosta-8,24(28)-dienol, ergosta-8,24(25)-dienol and 24-dimethyl ergosta-8,25(27)-dienol in a ratio of substrate/product of 7:3. The products distributed in a ratio of approximately 5:1:2:2 respectively Trypanosoma brucei ?
-
?

Synonyms

Synonyms Comment Organism
sterol C24-methyltransferase
-
Trypanosoma brucei
TbSMT
-
Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
assay at Trypanosoma brucei

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Trypanosoma brucei