Protein Variants | Comment | Organism |
---|---|---|
Y81A | Km (mM): 0.018 (zymosterol), 0.023 (26,27-dehydrozymosterol), kcat (1/sec): 0.00083 (zymosterol), 0.000116 (26,27-dehydrozymosterol), Ki (mM): 0.011 (26,27-dehydrozymosterol), 0.00019 (25-azalanosterol), 0.00013 (24(R,S),25-epiminolanosterol), 0.071 (ergosterol) | Saccharomyces cerevisiae |
Y81F | Km (mM): 0.017 (zymosterol), 0.026 (26,27-dehydrozymosterol), kcat (1/sec): 0.0108 (zymosterol), 0.0025 (26,27-dehydrozymosterol), Ki (mM): 0.010 (26,27-dehydrozymosterol), 0.00036 (25-azalanosterol), 0.00031 (24(R,S),25-epiminolanosterol), 0.082 (ergosterol) | Saccharomyces cerevisiae |
Y81I | Km (mM): 0.021 (zymosterol), 0.027 (26,27-dehydrozymosterol), kcat (1/sec): 0.00433 (zymosterol), 0.00025 (26,27-dehydrozymosterol), Ki (mM): 0.009 (26,27-dehydrozymosterol), 0.00020 (25-azalanosterol), 0.00016 (24(R,S),25-epiminolanosterol), 0.077 (ergosterol) | Saccharomyces cerevisiae |
Y81L | Km (mM): 0.018 (zymosterol), 0.024 (26,27-dehydrozymosterol), kcat (1/sec): 0.00416 (zymosterol), 0.00033 (26,27-dehydrozymosterol), Ki (mM): 0.011 (26,27-dehydrozymosterol), 0.00021 (25-azalanosterol), 0.00018 (24(R,S),25-epiminolanosterol), 0.078 (ergosterol) | Saccharomyces cerevisiae |
Y81V | Km (mM): 0.026 (zymosterol), 0.024 (26,27-dehydrozymosterol), kcat (1/sec): 0.00116 (zymosterol), 0.00011 (26,27-dehydrozymosterol), Ki (mM): 0.007 (26,27-dehydrozymosterol), 0.000195 (25-azalanosterol), 0.000145 (24(R,S),25-epiminolanosterol), 0.074 (ergosterol) | Saccharomyces cerevisiae |
Y81W | Km (mM): 0.017 (zymosterol), 0.023 (26,27-dehydrozymosterol), kcat (1/sec): 0.0133 (zymosterol), 0.0033 (26,27-dehydrozymosterol), Ki (mM): 0.010 (26,27-dehydrozymosterol), 0.00039 (25-azalanosterol), 0.00036 (24(R,S),25-epiminolanosterol), 0.088 (ergosterol) | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
24(R,S)-25-epiminolanosterol | - |
Saccharomyces cerevisiae | |
25-azalanosterol | - |
Saccharomyces cerevisiae | |
26,27-dehydrozymosterol | - |
Saccharomyces cerevisiae | |
ergosterol | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
Zymosterol | wild-type | Saccharomyces cerevisiae | |
0.017 | - |
Zymosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.017 | - |
Zymosterol | mutant Y81W | Saccharomyces cerevisiae | |
0.018 | - |
Zymosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.018 | - |
Zymosterol | mutant Y81L | Saccharomyces cerevisiae | |
0.021 | - |
Zymosterol | mutant Y81I | Saccharomyces cerevisiae | |
0.022 | - |
26,27-dehydrozymosterol | wild-type | Saccharomyces cerevisiae | |
0.023 | - |
26,27-dehydrozymosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.023 | - |
26,27-dehydrozymosterol | mutant Y81W | Saccharomyces cerevisiae | |
0.024 | - |
26,27-dehydrozymosterol | mutant Y81L | Saccharomyces cerevisiae | |
0.024 | - |
26,27-dehydrozymosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.026 | - |
26,27-dehydrozymosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.026 | - |
Zymosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.027 | - |
26,27-dehydrozymosterol | mutant Y81I | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type SMT is expressed and partially purified by anionic-exchange chromatography to generate ca. 85% pure Erg6p as determined by SDS-PAGE quantification of the target protein | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activity using 26,27-dehydrozymosterol as a substrate: wild-type 100%, mutant Y81F 300%, mutant Y81W 450%, mutant Y81I 25%, mutant Y81L 40%, mutant Y81V 15%, mutant Y81A 15% | Saccharomyces cerevisiae |
additional information | - |
relative activity using zymosterol as a substrate: wild-type 100%, mutant Y81F 92%, mutant Y81W 124%, mutant Y81I 32%, mutant Y81L 37%, mutant Y81V 8%, mutant Y81A 8% | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
26,27-dehydrozymosterol + S-adenosyl-L-methionine | in contrast to the results with zymosterol, product analysis of the 26,27-dehydrozymosterol assays shows that multiple products are produced that occurred in different ratios determined by HPLC-radiocounting of the non-saponifiable lipid fractions. In these analyses two major product classes are identified, a neutral C26 sterol monol and a pair of C26-oxygenated diols | Saccharomyces cerevisiae | ? + S-adenosyl-L-homocysteine | - |
? | |
zymosterol + S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae | fecosterol + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C24-methyltransferase | - |
Saccharomyces cerevisiae |
Erg6p | - |
Saccharomyces cerevisiae |
SMT | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001167 | - |
26,27-dehydrozymosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.0001167 | - |
26,27-dehydrozymosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.00025 | - |
26,27-dehydrozymosterol | mutant Y81I | Saccharomyces cerevisiae | |
0.0003333 | - |
26,27-dehydrozymosterol | mutant Y81L | Saccharomyces cerevisiae | |
0.0006667 | - |
26,27-dehydrozymosterol | wild-type | Saccharomyces cerevisiae | |
0.0008333 | - |
Zymosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.001167 | - |
Zymosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.0025 | - |
26,27-dehydrozymosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.003333 | - |
26,27-dehydrozymosterol | mutant Y81W | Saccharomyces cerevisiae | |
0.004167 | - |
Zymosterol | mutant Y81L | Saccharomyces cerevisiae | |
0.004333 | - |
Zymosterol | mutant Y81I | Saccharomyces cerevisiae | |
0.01 | - |
Zymosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.01083 | - |
Zymosterol | wild-type | Saccharomyces cerevisiae | |
0.01333 | - |
Zymosterol | mutant Y81W | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000025 | - |
24(R,S)-25-epiminolanosterol | wild-type | Saccharomyces cerevisiae | |
0.00003 | - |
25-azalanosterol | wild-type | Saccharomyces cerevisiae | |
0.00013 | - |
24(R,S)-25-epiminolanosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.000145 | - |
24(R,S)-25-epiminolanosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.000165 | - |
24(R,S)-25-epiminolanosterol | mutant Y81I | Saccharomyces cerevisiae | |
0.00018 | - |
24(R,S)-25-epiminolanosterol | mutant Y81L | Saccharomyces cerevisiae | |
0.00019 | - |
25-azalanosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.000195 | - |
25-azalanosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.000205 | - |
25-azalanosterol | mutant Y81I | Saccharomyces cerevisiae | |
0.00021 | - |
25-azalanosterol | mutant Y81L | Saccharomyces cerevisiae | |
0.00031 | - |
24(R,S)-25-epiminolanosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.00036 | - |
25-azalanosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.00036 | - |
24(R,S)-25-epiminolanosterol | mutant Y81W | Saccharomyces cerevisiae | |
0.00039 | - |
25-azalanosterol | mutant Y81W | Saccharomyces cerevisiae | |
0.00071 | - |
ergosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.000745 | - |
ergosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.00077 | - |
ergosterol | mutant Y81I | Saccharomyces cerevisiae | |
0.00078 | - |
ergosterol | mutant Y81L | Saccharomyces cerevisiae | |
0.00082 | - |
ergosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.00085 | - |
ergosterol | wild-type | Saccharomyces cerevisiae | |
0.00088 | - |
ergosterol | mutant Y81W | Saccharomyces cerevisiae | |
0.004 | - |
26,27-dehydrozymosterol | wild-type | Saccharomyces cerevisiae | |
0.007 | - |
26,27-dehydrozymosterol | mutant Y81V | Saccharomyces cerevisiae | |
0.009 | - |
26,27-dehydrozymosterol | mutant Y81I | Saccharomyces cerevisiae | |
0.01 | - |
26,27-dehydrozymosterol | mutant Y81F | Saccharomyces cerevisiae | |
0.01 | - |
26,27-dehydrozymosterol | mutant Y81W | Saccharomyces cerevisiae | |
0.011 | - |
26,27-dehydrozymosterol | mutant Y81A | Saccharomyces cerevisiae | |
0.011 | - |
26,27-dehydrozymosterol | mutant Y81L | Saccharomyces cerevisiae |