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Literature summary for 2.1.1.41 extracted from
- Mechanistic analysis of a multiple product sterol methyltransferase implicated in ergosterol biosynthesis in Trypanosoma brucei (2006), J. Biol. Chem., 281, 6290-6296.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | inhibition studies as a powerful approach to rational design of new anti-sleeping sickness chemotherapeutic drugs | Trypanosoma brucei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Escherichia coli HMS-174 cells | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 24(R,S),25-epiminolanosterol | - |
Trypanosoma brucei |  |
| 25-azalanosterol | - |
Trypanosoma brucei | |
| 26,27-dehydrozymosterol | - |
Trypanosoma brucei | |
| ergosterol | - |
Trypanosoma brucei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.047 | - |
Zymosterol | - |
Trypanosoma brucei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
4 * 40000, SDS-PAGE | Trypanosoma brucei |
| 160000 | - |
gel filtration | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | neither lanosterol nor other C4-methyl-containing sterols bind productively to the enzyme | Trypanosoma brucei | ? | - |
? | |
| zymosterol + S-adenosyl-L-methionine | C4-demethyl sterols with double bonds in the nucleus at positions 8, 7, or 5 are acceptable substrates | Trypanosoma brucei | fecosterol + S-adenosyl-L-homocysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 40000, SDS-PAGE | Trypanosoma brucei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SMT | - |
Trypanosoma brucei |
| sterol methyltransferase | - |
Trypanosoma brucei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Trypanosoma brucei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
Zymosterol | - |
Trypanosoma brucei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Trypanosoma brucei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.5 | - |
Trypanosoma brucei |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000039 | - |
25-azalanosterol | - |
Trypanosoma brucei | |
| 0.000049 | - |
24(R,S),25-epiminolanosterol | - |
Trypanosoma brucei | |
| 0.027 | - |
ergosterol | - |
Trypanosoma brucei | |
| 0.029 | - |
26,27-dehydrozymosterol | - |
Trypanosoma brucei | |
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Release 2023.1 (January 2023)
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