Any feedback?
Literature summary for 2.1.1.4 extracted from
- Caffeic acid O-methyltransferase is involved in the synthesis of melatonin by methylating N-acetylserotonin in Arabidopsis (2014), J. Pineal Res., 57, 219-227.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene comt, recombinant expression in Escherichia coli strain BL21(DE3), the N-terminal His-tagged AtCOMT is more highly expressed in soluble form than the C-terminal His-tagged AtCOMT | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an Arabidopsis thaliana comt knockout mutant (SALK_002373) with T-DNA inserted at the third exon. The comt mutant shows no detectable COMT transcript and exhibits a 20fold less COMT activity relative over the wild type, the phenotypes such as growth and fertility are indistinguishable from the wild-type plants | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.233 | - |
N-acetylserotonin | pH 7.8, 30°C | Arabidopsis thaliana |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | ? | - |
? | |
| additional information | Arabidopsis thaliana Col-0 | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | ? | - |
? | |
| S-adenosyl-L-methionine + N-acetylserotonin | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + melatonin | - |
? | |
| S-adenosyl-L-methionine + N-acetylserotonin | Arabidopsis thaliana Col-0 | - |
S-adenosyl-L-homocysteine + melatonin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9T003 | gene At4g35160 or AtASMT; gene AtASMT | - |
| Arabidopsis thaliana Col-0 | Q9T003 | gene At4g35160 or AtASMT; gene AtASMT | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| reombinant N-and C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatograpy | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | Arabidopsis thaliana | ? | - |
? | |
| additional information | caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines | Arabidopsis thaliana Col-0 | ? | - |
? | |
| S-adenosyl-L-methionine + N-acetylserotonin | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + melatonin | - |
? | |
| S-adenosyl-L-methionine + N-acetylserotonin | - |
Arabidopsis thaliana Col-0 | S-adenosyl-L-homocysteine + melatonin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ASMT | - |
Arabidopsis thaliana |
| N-acetylserotonin methyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
both recombinant N- and C-terminal fusions of enzyme AtCOMT exhibit the highest enzyme activities at pH 37°C | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
both recombinant N- and C-terminal fusions of enzyme AtCOMT exhibit the highest enzyme activities at pH 7.8 | Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.8 | both recombinant N- and C-terminal fusions of the enzyme, no activity at pH 5.4 | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | in vivo role played by the COMT for biosynthesizing melatonin | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
