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Literature summary for 2.1.1.383 extracted from
- MtcB, a member of the MttB superfamily from the human gut acetogen Eubacterium limosum, is a cobalamindependent carnitine demethylase (2020), J. Biol. Chem., 295, 11971-11981 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Eubacterium limosum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
L-carnitine | pH 7.2, 37°C | Eubacterium limosum |  |
| 1.34 | - |
cob(I)alamin | pH 7.2, 37°C | Eubacterium limosum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eubacterium limosum | A0A317RMF0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| gamma-butyrobetaine + cob(I)alamin | reaction rate is considerably lower than the rate of the enzyme with L-carnitine | Eubacterium limosum | methylcob(I)alamin + 4-(dimethylamino)butanoate | - |
? | |
| L-carnitine + Co(I)-MtqC | MtqC i.e. a corrinoid-binding protein | Eubacterium limosum | methyl-Co(I)-MtqC + L-norcarnitine | - |
? | |
| L-carnitine + cob(I)alamin | - |
Eubacterium limosum | methylcob(I)alamin + L-norcarnitine | - |
? | |
| additional information | recombinant MtcB methylates either cob(I)alamin or Co(I)-MtqC in the presence of L-carnitine and, to a much lesser extent, gamma-butyrobetaine. No methyl donors: choline, glycine betaine, proline betaine, trimethylamine, or tetramethylammonium | Eubacterium limosum | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C7955_10966 | - |
Eubacterium limosum |
| MtcB | - |
Eubacterium limosum |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Eubacterium limosum | MtcB constitutes 2.9 mol% of detected proteins from L-carnitine-grown cells. MtcB is not detectable in lactate-grown cells | additional information |
| Eubacterium limosum | expression is induced by grown on L-carnitine | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is a component of a system that transfers a methyl group from L-carnitine to tetrahydrofolate, as part of an L-carnitine degradation pathway | Eubacterium limosum |
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Release 2023.1 (January 2023)
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