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Literature summary for 2.1.1.37 extracted from
- Fluorescence-based high-throughput assay for human DNA (cytosine-5)-methyltransferase 1 (2010), Anal. Biochem., 401, 168-172.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | using a truncated and highly active form of human DNMT1 and a designed hemimethylated DNA substrate, a robust, efficient, and economical fluorescence assay is developped suitable for in vitro high-throughput screening of DNMT1 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELAT1-501 | to accelerate the rate of DNMT1 with the hemimethylated substrate, the 501 amino acids at the N terminus are deleted. Truncated DNMT1 exhibits a 4fold increased kcat/Km as compared with the full-length enzyme and does not require allosteric activation by hemimethylated DNA | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + S-adenosyl-L-methionine | a hemimethylated 5MeCpG target site is generated using Sau3AI restriction enzyme | Homo sapiens | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dnmt1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay carried out at room temperature | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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