Cloned (Comment) | Organism |
---|---|
construction of pDIM-N7 and pAR for expression of M.EcoHK31I fragments, construction of pDIM-N7 Tyr123-alpha and pAR beta-Tyr456 plasmids for combinatorial library, the recombinantly expressed M.EcoHK31I enzyme is functional in vivo when the two fragments are encoded as separate genes, overview | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | removal of 50 amino acids from the N-terminus of the beta fragment of M.EcoHK31I severely reduces but does not eliminate methylation activity in vivo. Design of the methylation target site and fusion of the a and betaDELTA42 fragments to zinc fingers | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | methylation specificity is affected by gene copy number and induction levels | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Escherichia coli | - |
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | - |
Escherichia coli | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
DNA + S-adenosyl-L-methionine | DNA methyltransferase M.EcoHK31I methylates the inner cytosine of 5'-YGGCCR-3' | Escherichia coli | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
additional information | site-based methylation is dependent on the relative position of the zinc finger binding sites and the target methylation site, methylation specificity for the target site also depends on zinc finger binding | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | DNA methyltransferase M.EcoHK31I is a natural heterodimer | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DNA methyltransferase | - |
Escherichia coli |
M.EcoHK31l | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |