Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-pyrimidinone | forms a part of inhibitor 1,2-dihydropyrimidin-2-one-5-methylene-(methylsulfonium)-adenosyl, bindng structure and mechanism, overview | Homo sapiens | |
5-aza-cytosine | - |
Homo sapiens | |
5-fluorocytosine | - |
Homo sapiens | |
[1,2-dihydropyrimidin-2-one]-5-methylene-(methylsulfonium)-adenosyl | with 2-pyrimidinone ring | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, Dnmt1 allosteric regulation mechanism, modelling, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | Homo sapiens | - |
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P26358 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | catalytic reaction mechanism of Dnmt1, allosteric regulation with fast equilibrium, and substrate specificity. Base-flipping and base-stacking are the key mechanism in control of enzymatic activity, detailed overview | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + S-adenosyl-L-methionine | - |
Homo sapiens | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
DNA + S-adenosyl-L-methionine | Dnmt1 methylates DNA at CG sites. Substrate binding and involved motifs in Dnmt1, overview | Homo sapiens | DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine | - |
? | |
additional information | no activity with single-stranded DNA with one Cm5 site, double-stranded fully methylated DNA, and poly(dA)-poly(T), pol(G), poly(dC)-poly(dG) or poly(dA-dT) | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Dnmt1 has a small catalytid C-terminal domain and a large regulatory domain at the N-terminal, the two domains are linked by 12 Gly-Lys repeats, structure, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cytosine carbon 5 DNA methyltransferase | - |
Homo sapiens |
cytosine DNA methyltransferase | - |
Homo sapiens |
Dnmt1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition by mechanism-based inhibitors and kinetics, detailed overview | Homo sapiens |