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Literature summary for 2.1.1.37 extracted from

  • Turnbull, J.F.; Adams, R.L.P.
    DNA methylase: purification from ascites cells and the effect of various DNA substrates on its activity (1976), Nucleic Acids Res., 3, 677-695.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.37

Inhibitors

Inhibitors Comment Organism Structure
NaCl incubation of native calf thymus DNA in presence of 40 mM NaCl results in 50% inhibition, more than 90% inhibition at 200 mM. With denatured calf thymus DNA, low concentrations of NaCl, up to 90 mM stimulate, 50% inhibition at 175 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 S-adenosyl-L-methionine
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl incubation of native calf thymus DNA in presence of 40 mM NaCl results in 50% inhibition, more than 90% inhibition at 200 mM. With denatured calf thymus DNA, low concentrations of NaCl, up to 90 mM stimulate, 50% inhibition at 175 mM Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE Mus musculus
240000
-
 gel filtration Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Krebs II ascites cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000107
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
 Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
S-adenosyl-L-methionine + DNA both native and denatured DNA are methylated, with calf thymus DNA the double stranded form is the better substrate but the enzyme preferentially methylates single stranded E. coli DNA even in the native preparation. Homologous ascites DNA can be methylated, but only to about 5% of the level of the best substrate, undermethylated mouse L929 cell DNA. DNA isolated from growing cells or tissues is a better substrate than DNA from normal liver or pancreas or from stationary cells Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Mus musculus