Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | incubation of native calf thymus DNA in presence of 40 mM NaCl results in 50% inhibition, more than 90% inhibition at 200 mM. With denatured calf thymus DNA, low concentrations of NaCl, up to 90 mM stimulate, 50% inhibition at 175 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
S-adenosyl-L-methionine | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | incubation of native calf thymus DNA in presence of 40 mM NaCl results in 50% inhibition, more than 90% inhibition at 200 mM. With denatured calf thymus DNA, low concentrations of NaCl, up to 90 mM stimulate, 50% inhibition at 175 mM | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Mus musculus |
240000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Krebs II ascites cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000107 | - |
- |
Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA | - |
Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + DNA | both native and denatured DNA are methylated, with calf thymus DNA the double stranded form is the better substrate but the enzyme preferentially methylates single stranded E. coli DNA even in the native preparation. Homologous ascites DNA can be methylated, but only to about 5% of the level of the best substrate, undermethylated mouse L929 cell DNA. DNA isolated from growing cells or tissues is a better substrate than DNA from normal liver or pancreas or from stationary cells | Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Mus musculus |