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Literature summary for 2.1.1.37 extracted from

  • Roy, P.H.; Weissbach, A.
    DNA methylase from HeLa cell nuclei (1975), Nucleic Acids Res., 2, 1669-1684.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
glycerol stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00325
-
S-adenosyl-L-methionine
-
Homo sapiens
0.086
-
Micrococcus luteus DNA containing cytosine
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
glycerol density gradient sedimentation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000013
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, stable for at least several weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + DNA
-
Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + DNA the enzyme is able to methylate homologues HeLa DNA, altough to a lesser extent than heterologous DNA. The enzyme can methylate single-stranded DNA three times higher than that of the corresponding double-stranded DNA, the 5' neighbor can be either G or C while the 3' neighbor is always G, enzyme can methylate poly(dG,dC) Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
?
S-adenosyl-L-methionine + Micrococcus luteus DNA containing cytosine
-
Homo sapiens S-adenosyl-L-homocysteine + Micrococcus luteus DNA containing 5-methylcytosine
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Homo sapiens