Cloned (Comment) | Organism |
---|---|
genes PAB0719 and PAB0760, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) | Pyrococcus abyssi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains an [Fe4S4] cluster, overview | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tRNA containing uridine at position 54 | Pyrococcus abyssi | - |
S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
genes PAB0719 and PAB0760 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tRNA containing uridine at position 54 | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54 | - |
? | |
S-adenosyl-L-methionine + tRNA containing uridine at position 54 | PAB0719 enzyme or PabTrmU54 displays an identical specificity to eucaryontal homologue TrmA, as it catalyses the in vitro formation of m5U-54 in tRNA | Pyrococcus abyssi | S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAB0719 enzyme | - |
Pyrococcus abyssi |
PabTrmU54 | - |
Pyrococcus abyssi |
RumA | - |
Pyrococcus abyssi |
RumA-type tRNA(uracil-54, C5)-methyltransferase | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pyrococcus abyssi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on | Pyrococcus abyssi |