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Literature summary for 2.1.1.35 extracted from

  • Johansson, M.J.O.; Bystr๖m, A.S.
    Dual function of the tRNA(m5U54)methyltransferase in tRNA maturation (2002), RNA, 8, 324-335.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.35

Cloned(Commentary)

Cloned (Comment) Organism
enzyme is encoded by the nonessential TRM2 gene Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C521A mutant without methyltransferase activity, putative nucleophilic Cys-521 may be involved in Cys-U54 bond formation Saccharomyces cerevisiae
G428D mutant without methyltransferase activity, G-428 is located at the presumed S-adenosyl-L-methionine binding site Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine54 in tRNA Saccharomyces cerevisiae role per se in tRNA maturation, stabilization of tRNA, early event in tRNA maturation: intron-containing pre-tRNAs contain m5U S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 several strains
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information absence of TRM2 gene encoding enzyme in strains with mutant forms of tRNASerCGA decreases the stability of tRNASerCGA and induces lethality Saccharomyces cerevisiae additional information
-
 ?
S-adenosyl-L-methionine + uridine54 in tRNA methylation of U54 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA nearly all tRNAs contain m5U54, initiators excluded ?
S-adenosyl-L-methionine + uridine54 in tRNA role per se in tRNA maturation, stabilization of tRNA, early event in tRNA maturation: intron-containing pre-tRNAs contain m5U Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
 ?

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae