KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values for several mutants of yeast tRNAPhe | Escherichia coli | |
0.0008 | - |
uracil54 in yeast tRNA-Phe | - |
Escherichia coli | |
0.0028 | - |
uracil54 in unmodified E. coli tRNA1-Val | - |
Escherichia coli | |
0.0037 | - |
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | |
0.005 | - |
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | catalytic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 16S rRNA | unmodified E. coli 16S rRNA can be methylated at U788 | Escherichia coli | S-adenosyl-L-homocysteine + 16S rRNA containing thymine | - |
? | |
S-adenosyl-L-methionine + uracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
? | |
S-adenosyl-L-methionine + uracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
? | |
S-adenosyl-L-methionine + uracil54 in unmodified E. coli tRNA1-Val | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified E. coli tRNA1-Val | - |
? | |
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe | - |
? | |
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe | - |
Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe | - |
r | |
S-adenosyl-L-methionine + uridine54 in tRNA | tRNA recognition reside in the 3-dimensional structure of the 7-member T-loop rather than in its primary structure, base composition of the T-stem is also unimportant, only U54 and C56 are essential for activity, tRNA binding causes a conformational change of tRNA to expose the modification site in the T-arm | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | substrate consensus sequence for the T-arm of tRNA consists of a 2-5 base-pair stem and a 7-base loop | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: unmodified yeast tRNAPhe | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: tRNA from E. coli | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptors: E. coli tRNA1Val, 17-mer analog of the T-arm from tRNA1Val | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | tRNA recognition | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methylation of U54 | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | values for several mutants of yeast tRNAPhe | Escherichia coli | |
0.0015 | - |
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | |
0.0333 | - |
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val | - |
Escherichia coli | |
0.048 | - |
uracil54 in unmodified E. coli tRNA1-Val | - |
Escherichia coli | |
0.0917 | - |
uracil54 in yeast tRNA1-Phe | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |