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Literature summary for 2.1.1.35 extracted from

  • Gu, X.; Ivanetich, K.M.; Santi, D.V.
    Recognition of the T-arm of tRNA by tRNA (m5U54)-methyltransferase is not sequence specific (1996), Biochemistry, 35, 11652-11659.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information values for several mutants of yeast tRNAPhe Escherichia coli
0.0008
-
uracil54 in yeast tRNA-Phe
-
Escherichia coli
0.0028
-
uracil54 in unmodified E. coli tRNA1-Val
-
Escherichia coli
0.0037
-
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli
0.005
-
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA catalytic mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 16S rRNA unmodified E. coli 16S rRNA can be methylated at U788 Escherichia coli S-adenosyl-L-homocysteine + 16S rRNA containing thymine
-
?
S-adenosyl-L-methionine + uracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
?
S-adenosyl-L-methionine + uracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
?
S-adenosyl-L-methionine + uracil54 in unmodified E. coli tRNA1-Val
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in unmodified E. coli tRNA1-Val
-
?
S-adenosyl-L-methionine + uracil54 in yeast tRNA-Phe
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA-Phe
-
?
S-adenosyl-L-methionine + uracil54 in yeast tRNA1-Phe
-
Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil54 in yeast tRNA1-Phe
-
r
S-adenosyl-L-methionine + uridine54 in tRNA tRNA recognition reside in the 3-dimensional structure of the 7-member T-loop rather than in its primary structure, base composition of the T-stem is also unimportant, only U54 and C56 are essential for activity, tRNA binding causes a conformational change of tRNA to expose the modification site in the T-arm Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA substrate consensus sequence for the T-arm of tRNA consists of a 2-5 base-pair stem and a 7-base loop Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: unmodified yeast tRNAPhe Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptor: tRNA from E. coli Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA methyl group acceptors: E. coli tRNA1Val, 17-mer analog of the T-arm from tRNA1Val Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA tRNA recognition Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?
S-adenosyl-L-methionine + uridine54 in tRNA methylation of U54 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information values for several mutants of yeast tRNAPhe Escherichia coli
0.0015
-
uracil54 in 11mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli
0.0333
-
uracil54 in 17mer of the T-arm of E. coli tRNA1-Val
-
Escherichia coli
0.048
-
uracil54 in unmodified E. coli tRNA1-Val
-
Escherichia coli
0.0917
-
uracil54 in yeast tRNA1-Phe
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Escherichia coli