Cloned (Comment) | Organism |
---|---|
trmA gene encodes enzyme, gene is essential for the organism | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
native enzyme form, sucrose density gradient centrifugation | Escherichia coli |
54000 | - |
TrmA/RNA complex, sucrose density gradient centrifugation | Escherichia coli |
62000 | - |
x * 54000, first TrmA/RNA-complex, x * 62000, second TrmA/RNA-complex, x * 42000, native enzyme form, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli | in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | Escherichia coli 1100 | in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
3 enzyme forms: native protein and 2 different TrmA/RNA complexes | - |
Escherichia coli 1100 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3 enzyme forms | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + uracil54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil54 in tRNA | catalytic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is present as 42 kDa native protein or as 2 different covalent TrmA/RNA complexes, with TrmA as a 54 kDa and a 62 kDa polypeptide, respectively, RNA is a subset of undermodified tRNA species as well as the 3Â’ terminus of 16S rRNA, RNA does not affect enzyme activity | Escherichia coli | additional information | - |
? | |
additional information | enzyme is present as 42 kDa native protein or as 2 different covalent TrmA/RNA complexes, with TrmA as a 54 kDa and a 62 kDa polypeptide, respectively, RNA is a subset of undermodified tRNA species as well as the 3Â’ terminus of 16S rRNA, RNA does not affect enzyme activity | Escherichia coli 1100 | additional information | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | enzyme transfers methyl group to uridine in position 54 of the TPsi-loop of all E. coli tRNA species, requires the TPsi-arm and, most specifically, the bases U54-U55-C56-Pu57-A58 for tRNA recognition | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: tRNA from E. coli | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone | Escherichia coli | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? | |
S-adenosyl-L-methionine + uridine54 in tRNA | enzyme transfers methyl group to uridine in position 54 of the TPsi-loop of all E. coli tRNA species, requires the TPsi-arm and, most specifically, the bases U54-U55-C56-Pu57-A58 for tRNA recognition | Escherichia coli 1100 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | methyl group acceptor: tRNA from E. coli | Escherichia coli 1100 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | formation of 5-methyluridine | ? | |
S-adenosyl-L-methionine + uridine54 in tRNA | in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone | Escherichia coli 1100 | S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, first TrmA/RNA-complex, x * 62000, second TrmA/RNA-complex, x * 42000, native enzyme form, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |