Crystallization (Comment) | Organism |
---|---|
in complex with S-adenosyl-L-methionine | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E124A | 13fold increase in Km-value | Thermus thermophilus |
I142A | kinetic parameters comparable to wild-type | Thermus thermophilus |
L101A | 13fold increase in Km-value | Thermus thermophilus |
L151A | 5fold increase in Km-value | Thermus thermophilus |
M144A | 15fold increase in Km-value | Thermus thermophilus |
N152A | 80fold increase in Km-value | Thermus thermophilus |
R41A | no enzymic activity | Thermus thermophilus |
R41K | 30fold increase in Km-value | Thermus thermophilus |
S150A | large increase in Km-value | Thermus thermophilus |
T99A | small increase in Km-value | Thermus thermophilus |
V128A | small increase in Km-value | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant I142A | Thermus thermophilus | |
0.01 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, wild-type | Thermus thermophilus | |
0.014 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant V128A | Thermus thermophilus | |
0.017 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant T99A | Thermus thermophilus | |
0.056 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant L151A | Thermus thermophilus | |
0.13 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant L101A | Thermus thermophilus | |
0.15 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant M144A | Thermus thermophilus | |
0.3 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant R41K | Thermus thermophilus | |
0.8 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant N152A | Thermus thermophilus | |
1.7 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant S150A | Thermus thermophilus | |
28 | - |
S-adenosyl-L-methionine | pH 7.6, 50°C, mutant E124A | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q9FAC4 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | mechanism, upon binding of guanine 18, a RNA main chain phosphate group activates R41, which then deprotonates the 2'-OH-group for methylation | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + tRNA | - |
Thermus thermophilus | S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanine | - |
? |