Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + guanosine18 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | substrate recognition sites and mechanism | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus | ? | - |
? | |
additional information | specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet | Thermus thermophilus | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methylation at 2'-OH specifically of unmodified G18 of the ribose in the D-loop of tRNA | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptor from yeast: tRNAPhe | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanosine18 in tRNA | methyl group acceptors from E. coli: methyl deficient tRNATyr, tRNAPhe, tRNAfMet | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in tRNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
yeast tRNAPhe | Thermus thermophilus |
65 | - |
Escherichia coli tRNAfMet | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermus thermophilus |