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Literature summary for 2.1.1.33 extracted from

  • Okamoto, M.; Fujiwara, M.; Hori, M.; Okada, K.; Yazama, F.; Konishi, H.; Xiao, Y.; Qi, G.; Shimamoto, F.; Ota, T.; Temme, A.; Tatsuka, M.
    tRNA modifying enzymes, NSUN2 and METTL1, determine sensitivity to 5-fluorouracil in HeLa cells (2014), PLoS Genet., 10, e1004639.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.33

Application

Application Comment Organism
pharmacology enzymes NSUN2 and METTL1 are implicated in 5-fluorouracil sensitivity in HeLa cells. Interfering with methylation of tRNAs might provide a promising rationale to improve 5-fluorouracil chemotherapy of cancer Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information combined knockdown of NSUN2, EC 2.1.1.203, and METTL1 in HeLa cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein METTL1 is phosphorylated at Ser27 by Akt/protein kinase Balpha to inhibit its enzymatic activity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-
additional information simultaneous overexpression of NSUN2, EC 2.1.1.203, and METTL1 is widely observed among human cancers Homo sapiens
-

Synonyms

Synonyms Comment Organism
methyltransferase like 1
-
 Homo sapiens
METTL1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
evolution nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzymes, NSUN2 and METTL1, are mammalian orthologues of yeast Trm4 and Trm8, which are required for protecting tRNA against tRNA decay Homo sapiens
malfunction combined knockdown of NSUN2, EC 2.1.1.203, and METTL1 in HeLa cells drastically potentiate sensitivity of cells to 5-fluorouracil, but does not affect cisplatin- and paclitaxel-induced cytotoxicity, synergistic effects of NSUN2 and METTL1 double knockdown, which causes rapid tRNA(ValAAC) degradation induced by destabilizing 5-fluorouracil Homo sapiens
physiological function Nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzyme METTL1 is a mammalian orthologues of yeast Trm8, which is required for protecting tRNA against tRNA decay Homo sapiens