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Literature summary for 2.1.1.33 extracted from

  • Alexandrov, A.; Grayhack, E.J.; Phizicky, E.M.
    tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p (2005), RNA, 11, 821-830.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information strains lacking Trm8p/Trm82p tRNA m7G methyltransferase, are temperature-sensitive in synthetic media containing glycerol, trm82-delta strain shows reduced levels of Trm8p, but not of its mRNA, the growth phenotype of trm8 mutants correlates with lack of tRNA m7G methyltransferase activity in vitro and in vivo, bacterial Trm8p orthologs complement the trm8-delta trm82-delta growth phenotype and restore m7G-methyltransferase activity in vitro and in vivo Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine46 in tRNA Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q03774 and Q12009 Q03774: subunit Trm82, Q2009: subunit Trm8
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine46 in tRNA
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
 ?

General Information

General Information Comment Organism
physiological function Trm8p is required for m7G modification. Trm82p is required to maintain cellular levels of Trm8p and to stabilize Trm8p in an active conformation Saccharomyces cerevisiae