Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Methylthioinosine | activates at 0.05-0.2 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
S-Inosylhomocysteine | activates at 0.05-0.2 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
General Stability | Organism |
---|---|
freezing inactivates | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylthioadenosine | competitive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
Methylthioinosine | weak competitive inhibitor, above 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
additional information | not inhibited by butylthioadenosine, thioethanoladenosine, isobutylthioadenosine | Salmonella enterica subsp. enterica serovar Typhimurium | |
S-Adenosyl-D-homocysteine | competitive inhibitor, L-enantiomer more potent than D-enantiomer by 2 orders of magnitude | Salmonella enterica subsp. enterica serovar Typhimurium | |
S-adenosyl-DL-homocysteine | competitive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
S-adenosyl-L-cysteine | competitive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
S-adenosyl-L-homocysteine | potent competitive product inhibition, L-enantiomer more potent than D-enantiomer by 2 orders of magnitude | Salmonella enterica subsp. enterica serovar Typhimurium | |
S-Inosylhomocysteine | weak competitive inhibitor, above 0.5 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | Salmonella enterica subsp. enterica serovar Typhimurium | specific enzyme involved in the maturation of tRNA | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
wild-type strain TA 265 | - |
Purification (Comment) | Organism |
---|---|
partial purification by phosphocellulose column | Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNA | methyl group acceptor: undermethylated tRNA from Salmonella typhimurium CS 14, NH2- and COOH-group of S-adenosyl-L-methionine as well as the length of hydrocarbon chain connecting them to the aromatic ring of adenosine, chirality at the alpha-C atom and NH2-group of adenosine are important for substrate binding to enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine46 in tRNA | specific enzyme involved in the maturation of tRNA | Salmonella enterica subsp. enterica serovar Typhimurium | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
S-adenosyl-L-homocysteine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.064 | - |
S-adenosyl-DLhomocysteine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.46 | - |
methylthioadenosine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.55 | - |
S-adenosyl-L-cysteine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.84 | - |
S-Adenosyl-D-homocysteine | - |
Salmonella enterica subsp. enterica serovar Typhimurium |