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Literature summary for 2.1.1.27 extracted from

  • Mann, J.D.; Mudd, S.H.
    Alkaloids and plant metabolism (1963), J. Biol. Chem., 238, 381-385.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Homotyramine
-
Hordeum vulgare
additional information EDTA is not inhibitory Hordeum vulgare
N-Methyltyramine
-
Hordeum vulgare
p-chloromercuribenzoate strong Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
tyramine
-
Hordeum vulgare
0.03
-
S-adenosyl-L-methionine
-
Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Hordeum vulgare involved in hordenine biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
L. var. Betzes, barley
-

Oxidation Stability

Oxidation Stability Organism
sulfhydryl compound necessary for stabilization Hordeum vulgare

Purification (Commentary)

Purification (Comment) Organism
partial Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
root of seedling Hordeum vulgare
-

Storage Stability

Storage Stability Organism
-18°C, addition of sucrose to a final concentration of 0.2 M, several weeks Hordeum vulgare
4°C, pH 7.0 and 5 mM 2-mercaptoethanol, several days Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorophenethylamine + S-adenosyl-L-methionine 29% of activity with tyramine Hordeum vulgare ?
-
?
beta-phenethanolamine + S-adenosyl-L-methionine 23% of activity with tyramine Hordeum vulgare ?
-
?
additional information involved in hordenine biosynthesis Hordeum vulgare ?
-
?
S-adenosyl-L-methionine + alpha-methyltyramine i.e. paredrine, 53% of activity with tyramine Hordeum vulgare ?
-
?
S-adenosyl-L-methionine + tyramine conversion rate is higher with (-)-L-diastereoisomer of methionine Hordeum vulgare N-methyltyramine + S-adenosyl-L-homocysteine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
assay at Hordeum vulgare

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
heat-labile: 70% loss of activity after 10 min Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor requirement Hordeum vulgare