Literature summary for 2.1.1.232 extracted from

Shimizu, T.; Lin, F.; Hasegawa, M.; Okada, K.; Nojiri, H.; Yamane, H.
Purification and identification of naringenin 7-O-methyltransferase, a key enzyme in biosynthesis of flavonoid phytoalexin sakuranetin in rice (2012), J. Biol. Chem., 287, 19315-19325.

Search for more literature for 2.1.1.232

Cloned(Commentary)

Cloned (Comment)	Organism
gene Os12g0240900, DNA and amino acid sequence determination and analysis, expression of GST-tagged NOMT in Escherichia coli strain Rosetta II (DE3)	Oryza sativa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetic analysis, overview	Oryza sativa
0.0019	-	(2S)-naringenin	pH 8.5, 30°C, purified recombinant GST-tagged NOMT	Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + (2S)-naringenin	Oryza sativa	-	S-adenosyl-L-homocysteine + (2S)-sakuranetin	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	I2FFE9	var. japonica and cv. Nipponbare, gene Os12g0240900	-

Purification (Commentary)

Purification (Comment)	Organism
purification of native enzyme 400fold from caffeic acid 3-O-methyltransferase-deficient oscomt1 mutant rice plant leaves induced with UV-light by ammonium sulfate fractionation and adenosine affinity chromatography, recombinant GST-tagged NOMT from Escherichia coli strain Rosetta II (DE3) by glutathione affinity chromatography and ultrafiltration	Oryza sativa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Oryza sativa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0036	-	purified NOMT, pH 8.5, 28°C	Oryza sativa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme is also active on kaempferol, apigenin, luteolin, liquiritigenin, and quercetin	Oryza sativa	?	-	?
S-adenosyl-L-methionine + (2S)-naringenin	-	Oryza sativa	S-adenosyl-L-homocysteine + (2S)-sakuranetin	-	?
S-adenosyl-L-methionine + (2S)-naringenin	-	Oryza sativa	S-adenosyl-L-homocysteine + (2S)-sakuranetin	mass spectrometric and chiral analysis of sakuranetin product	?

Synonyms

Synonyms	Comment	Organism
NOMT	-	Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
28	30	assay at	Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
25	-	(2S)-naringenin	pH 8.5, 30°C, purified recombinant GST-tagged NOMT	Oryza sativa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Oryza sativa

Expression

Organism	Comment	Expression
Oryza sativa	enzyme expression is inducible by by ultraviolet irradiation, CuCl2 treatment, jasmonic acid treatment, and infection by phytopathogens, e.g. fungus Magnaporthe oryzae	up

General Information

General Information	Comment	Organism
metabolism	naringenin 7-O-methyltransferase is a key enzyme in biosynthesis of flavonoid phytoalexin sakuranetin in rice	Oryza sativa
additional information	sakuranetin has anti-inflammatory activity, anti-mutagenic activity, anti-pathogenic activities against Helicobacter pylori, Leishmania, and Trypanosoma and contributes to the maintenance of glucose homeostasis in animals	Oryza sativa
physiological function	naringenin 7-O-methyltransferase is a key enzyme in biosynthesis of major flavonoid phytoalexin sakuranetin in rice, sakuranetin is a useful compound as a plant antibiotic and a potential pharmaceutical agent	Oryza sativa

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Release 2023.1 (January 2023)