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Literature summary for 2.1.1.232 extracted from

  • Malla, S.; Koffas, M.A.; Kazlauskas, R.J.; Kim, B.G.
    Production of 7-O-methyl aromadendrin, a medicinally valuable flavonoid, in Escherichia coli (2012), Appl. Environ. Microbiol., 78, 684-694.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin, which is important in medicinal applications, via sakuranetin from 4-coumaric acid in Escherichia coli, overview Streptomyces avermitilis

Cloned(Commentary)

Cloned (Comment) Organism
subcloning in Escherichia coli strain DH5alpha, recombinant expression in Arabidopsis thaliana Streptomyces avermitilis

Protein Variants

Protein Variants Comment Organism
additional information the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin via sakuranetin from 4-coumaric acid in Escherichia coli, overview Streptomyces avermitilis
additional information production of 7-O-methyl aromadendrin in Escherichia coli strain BL21(DE3) recombinantly expressing the genes of several origins encoding enzymes 4-coumarate–coenzyme A ligase from Petroselinum crispum, chalcone synthase from Petunia hybrida, and chalcone flavanone isomerase from Medicago sativa to produce (2S)-naringenin, and further genes encoding flavanone-3-hydroxylase from Arabidopsis thaliana and the 7-O-methyltransferase from Streptomyces avermitilis to transform (2S)-naringenin into 7-O-methyl aromadendrin via dihydrokaempferol and (2S)-sakuranetin, method and pathway, overview Streptomyces avermitilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (2S)-naringenin Streptomyces avermitilis
-
S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
?
S-adenosyl-L-methionine + (2S)-naringenin Streptomyces avermitilis MA-4680
-
S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
?
S-adenosyl-L-methionine + dihydrokaempferol Streptomyces avermitilis
-
S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?
S-adenosyl-L-methionine + dihydrokaempferol Streptomyces avermitilis MA-4680
-
S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q79ZE8
-
-
Streptomyces avermitilis Q79ZE8 7-O-methyltransferase; gene SaOMT or SAV_2382
-
Streptomyces avermitilis MA-4680 Q79ZE8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (2S)-naringenin
-
Streptomyces avermitilis S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
?
S-adenosyl-L-methionine + (2S)-naringenin
-
Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
?
S-adenosyl-L-methionine + dihydrokaempferol
-
Streptomyces avermitilis S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?
S-adenosyl-L-methionine + dihydrokaempferol
-
Streptomyces avermitilis MA-4680 S-adenosyl-L-homocysteine + 7-O-methyl aromadendrin 7-O-methyl aromadendrin is an aglycone moiety of one of the important flavonoid-glycosides found in several plants with various medicinal applications ?

Synonyms

Synonyms Comment Organism
7-O-methyltransferase
-
Streptomyces avermitilis
SaOMT
-
Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Streptomyces avermitilis