Crystallization (Comment) | Organism |
---|---|
structure of RlmCD in complex with its cofactor and the RNA substrate containing U747 at 2.00 A or U1939 at 3.10 A. The side-chain rearrangement of F145 displays an unusual mechanism through which RlmCD can discriminate between U747- and U1939-containing RNA substrate by switching the intermolecular aromatic stacking between protein and RNA on/off | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D381A | 2% residual activity towards U747 RNA substrate, 40% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 | Streptococcus pneumoniae |
F145A | 5% residual activity towards U747 RNA substrate, 120% activity towards U1939 RNA substrate, reaction of EC 2.11.190 | Streptococcus pneumoniae |
H151A | 5% residual activity towards U747 RNA substrate, 55% activity towards U1939 RNA substrate, reaction of EC 2.11.190 | Streptococcus pneumoniae |
N249A | 35% residual activity towards U747 RNA substrate, 55% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 | Streptococcus pneumoniae |
Q162A | 18% residual activity towards U747 RNA substrate, 160% activity towards U1939 RNA substrate, reaction of EC 2.11.190 | Streptococcus pneumoniae |
Q283A | 8% residual activity towards U747 RNA substrate, 50% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 | Streptococcus pneumoniae |
R127A | 8% residual activity towards U747 RNA substrate, 2% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190 | Streptococcus pneumoniae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptococcus pneumoniae | enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 | ? | - |
- |
|
additional information | Streptococcus pneumoniae ATCC BAA-334 | enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | Q97R12 | introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4 | - |
Streptococcus pneumoniae ATCC BAA-334 | Q97R12 | introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 | Streptococcus pneumoniae | ? | - |
- |
|
additional information | enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 | Streptococcus pneumoniae | ? | - |
- |
|
additional information | enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190 | Streptococcus pneumoniae ATCC BAA-334 | ? | - |
- |
|
additional information | enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190 | Streptococcus pneumoniae ATCC BAA-334 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
RlmCD | - |
Streptococcus pneumoniae |
SP_1029 | - |
Streptococcus pneumoniae |