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Literature summary for 2.1.1.189 extracted from

  • Jiang, Y.; Li, F.; Wu, J.; Shi, Y.; Gong, Q.
    Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCD (2017), PLoS ONE, 12, e0185226 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.189

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of apo-form RlmCD and its complex with SAH. A long linker in the central domain of RlmCD is the key factor in determining its substrate selectivity Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information both full-length RlmCD and its shorter construct RlmCDs (residue 1-454) exhibit strong U74 methyltransferase activity when compared with the glutamine mutant of E443 Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4
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Streptococcus pneumoniae ATCC BAA-334 Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation Streptococcus pneumoniae ?
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additional information replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation Streptococcus pneumoniae ATCC BAA-334 ?
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S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3' 18-mer RNA analogue of the 23S rRNA helix 35 Streptococcus pneumoniae S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3'
-
 ?
S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3' 18-mer RNA analogue of the 23S rRNA helix 35 Streptococcus pneumoniae ATCC BAA-334 S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3'
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 ?

Synonyms

Synonyms Comment Organism
RlmCD
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 Streptococcus pneumoniae
SP_1029
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 Streptococcus pneumoniae