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Literature summary for 2.1.1.160 extracted from

  • Mohanpuria, P.; Kumar, V.; Ahuja, P.S.; Yadav, S.K.
    Producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA (2011), Plant Mol. Biol., 76, 523-534.
    View publication on PubMed
Search for more literature for 2.1.1.160

Application

Application Comment Organism
food industry producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA Camellia sinensis

Cloned(Commentary)

Cloned (Comment) Organism
semiquantitative-PCR for caffeine synthase transcript expression analysis Camellia sinensis

Protein Variants

Protein Variants Comment Organism
additional information isolation of a partial fragment of caffeine synthase from cv. Kangra jat and usage for design an RNAi construct, pFGC1008-CS, detection of siRNAs specific to caffeine synthase in transgenic plants. Transformation of somatic embryos with the developed construct using a biolistic method. Transformed somatic embryos show reduction in the levels of caffeine synthase transcript expression as well as in caffeine content. Transgenic plants generated from the transformed somatic embryos show a significant suppression of caffeine synthase transcript expression and also a reduction of 44-61% in caffeine and 46-67% in theobromine contents as compared to the controls Camellia sinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis
-
 S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
 ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis
-
 cv. Kangra jat
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Camellia sinensis
-
seedling
-
 Camellia sinensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
S-adenosyl-L-methionine + 7-methylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine
-
 ?

Synonyms

Synonyms Comment Organism
3, 7-dimethylxanthine methyltransferase
-
 Camellia sinensis
7-N-methylxanthine methyltransferase
-
 Camellia sinensis

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Camellia sinensis

General Information

General Information Comment Organism
malfunction post-transcriptional silencing of caffeine synthase mRNA leads to reduced caffeine content in tea leaves Camellia sinensis
metabolism caffeine synthase is a bifunctional enzyme that catalyzes the last two steps of caffeine biosynthesis in tea as 7-N-methylxanthine methyltransferase and 3,7-dimethylxanthine methyltransferase Camellia sinensis