Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium marinum |
Crystallization (Comment) | Organism |
---|---|
the cocrystal structure of with S-adenosyl-L-homocysteine and octanoate shows that the substrate-binding site is largely localized within the alpha-helical domain that caps the Rossmann-like fold domain. Aliphatic and aromatic residues encircle the acyl chain of the fatty acid substrate, including Trp155, Met214, Phe222, Tyr224, Val256, Phe311, and Leu316. In the cocrystal structure with 3-hydroxydecanoate, only the S enantiomer is bound in the active site | Mycobacterium marinum |
Protein Variants | Comment | Organism |
---|---|---|
Q154A | 17fold increaseof the KM with octanoate as a substrate, and 5fold increase of the KM for 3-hydroxydecanoate | Mycobacterium marinum |
Q31A | complete loss of activtiy | Mycobacterium marinum |
W155F | near 1000fold decrease in catalytic efficiency | Mycobacterium marinum |
Y24F | 30fold decrease in catalytic efficiency | Mycobacterium marinum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0082 | - |
Decanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.0255 | - |
S-adenosyl-L-methionine | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.201 | - |
3-hydroxydecanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.308 | - |
octanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
1.03 | - |
3-hydroxydecanoic acid | mutant Q31A, pH 7.8, 37°C | Mycobacterium marinum | |
2.039 | - |
3-hydroxyoctanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
5.24 | - |
octanoic acid | mutant Q31A, pH 7.8, 37°C | Mycobacterium marinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium marinum | B2HHT4 | - |
- |
Mycobacterium marinum ATCC BAA-535 | B2HHT4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxydecanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine | - |
? | |
3-hydroxydecanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum ATCC BAA-535 | methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine | - |
? | |
3-hydroxyoctanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine | - |
? | |
3-hydroxyoctanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum ATCC BAA-535 | methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine | - |
? | |
decanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl decanoate + S-adenosyl-L-homocysteine | - |
? | |
decanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum ATCC BAA-535 | methyl decanoate + S-adenosyl-L-homocysteine | - |
? | |
octanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum | methyl octanoate + S-adenosyl-L-homocysteine | - |
? | |
octanoic acid + S-adenosyl-L-methionine | - |
Mycobacterium marinum ATCC BAA-535 | methyl octanoate + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAMT | - |
Mycobacterium marinum |
MMAR_3356 | - |
Mycobacterium marinum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
3-hydroxydecanoic acid | mutant Q31A, pH 7.8, 37°C | Mycobacterium marinum | |
0.147 | - |
octanoic acid | mutant Q31A, pH 7.8, 37°C | Mycobacterium marinum | |
0.244 | - |
Decanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.461 | - |
octanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.488 | - |
3-hydroxyoctanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.598 | - |
S-adenosyl-L-methionine | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.599 | - |
3-hydroxydecanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
octanoic acid | mutant W155F, pH 7.8, 37°C | Mycobacterium marinum | |
0.0031 | - |
3-hydroxydecanoic acid | mutant W155F, pH 7.8, 37°C | Mycobacterium marinum | |
0.028 | - |
octanoic acid | mutant Q31A, pH 7.8, 37°C | Mycobacterium marinum | |
0.041 | - |
3-hydroxydecanoic acid | mutant Q31A, pH 7.8, 37°C | Mycobacterium marinum | |
0.239 | - |
3-hydroxyoctanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
0.824 | - |
S-adenosyl-L-methionine | mutant Y24F, pH 7.8, 37°C | Mycobacterium marinum | |
1.46 | - |
octanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
2.98 | - |
3-hydroxydecanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
23.45 | - |
S-adenosyl-L-methionine | wild-type, pH 7.8, 37°C | Mycobacterium marinum | |
29.76 | - |
Decanoic acid | wild-type, pH 7.8, 37°C | Mycobacterium marinum |