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Literature summary for 2.1.1.15 extracted from

  • Petronikolou, N.; Nair, S.K.
    Biochemical studies of mycobacterial fatty acid methyltransferase: A catalyst for the enzymatic production of biodiesel (2015), Chem. Biol., 22, 1480-1490.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Mycobacterium marinum

Crystallization (Commentary)

Crystallization (Comment) Organism
the cocrystal structure of with S-adenosyl-L-homocysteine and octanoate shows that the substrate-binding site is largely localized within the alpha-helical domain that caps the Rossmann-like fold domain. Aliphatic and aromatic residues encircle the acyl chain of the fatty acid substrate, including Trp155, Met214, Phe222, Tyr224, Val256, Phe311, and Leu316. In the cocrystal structure with 3-hydroxydecanoate, only the S enantiomer is bound in the active site Mycobacterium marinum

Protein Variants

Protein Variants Comment Organism
Q154A 17fold increaseof the KM with octanoate as a substrate, and 5fold increase of the KM for 3-hydroxydecanoate Mycobacterium marinum
Q31A complete loss of activtiy Mycobacterium marinum
W155F near 1000fold decrease in catalytic efficiency Mycobacterium marinum
Y24F 30fold decrease in catalytic efficiency Mycobacterium marinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0082
-
Decanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
0.0255
-
S-adenosyl-L-methionine wild-type, pH 7.8, 37°C Mycobacterium marinum
0.201
-
3-hydroxydecanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
0.308
-
octanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
1.03
-
3-hydroxydecanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
2.039
-
3-hydroxyoctanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
5.24
-
octanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum

Organism

Organism UniProt Comment Textmining
Mycobacterium marinum B2HHT4
-
-
Mycobacterium marinum ATCC BAA-535 B2HHT4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxydecanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine
-
?
3-hydroxydecanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum ATCC BAA-535 methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine
-
?
3-hydroxyoctanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine
-
?
3-hydroxyoctanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum ATCC BAA-535 methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine
-
?
decanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum methyl decanoate + S-adenosyl-L-homocysteine
-
?
decanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum ATCC BAA-535 methyl decanoate + S-adenosyl-L-homocysteine
-
?
octanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum methyl octanoate + S-adenosyl-L-homocysteine
-
?
octanoic acid + S-adenosyl-L-methionine
-
Mycobacterium marinum ATCC BAA-535 methyl octanoate + S-adenosyl-L-homocysteine
-
?

Synonyms

Synonyms Comment Organism
FAMT
-
Mycobacterium marinum
MMAR_3356
-
Mycobacterium marinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
3-hydroxydecanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
0.147
-
octanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
0.244
-
Decanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
0.461
-
octanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
0.488
-
3-hydroxyoctanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
0.598
-
S-adenosyl-L-methionine wild-type, pH 7.8, 37°C Mycobacterium marinum
0.599
-
3-hydroxydecanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0014
-
octanoic acid mutant W155F, pH 7.8, 37°C Mycobacterium marinum
0.0031
-
3-hydroxydecanoic acid mutant W155F, pH 7.8, 37°C Mycobacterium marinum
0.028
-
octanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
0.041
-
3-hydroxydecanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
0.239
-
3-hydroxyoctanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
0.824
-
S-adenosyl-L-methionine mutant Y24F, pH 7.8, 37°C Mycobacterium marinum
1.46
-
octanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.98
-
3-hydroxydecanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
23.45
-
S-adenosyl-L-methionine wild-type, pH 7.8, 37°C Mycobacterium marinum
29.76
-
Decanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum