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Literature summary for 2.1.1.148 extracted from

  • Wang, K.; Wang, Q.; Chen, J.; Chen, L.; Jiang, H.; Shen, X.
    Crystal structure and enzymatic characterization of thymidylate synthase X from Helicobacter pylori strain SS1 (2011), Protein Sci., 20, 1398-1410.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NADPH 0.1 mM NADPH saturates the enzymatic reaction Helicobacter pylori

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M sodium chloride, 0.1 M MES, pH 6.0, and 20% (w/v) PEG2000 monomethyl ether Helicobacter pylori

Inhibitors

Inhibitors Comment Organism Structure
NADPH excess NADPH decreases enzyme activity Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0153
-
dUMP in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C Helicobacter pylori
0.35
-
5,10-methylenetetrahydrofolate in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
4 * 25000, SDS-PAGE Helicobacter pylori
25020
-
4 * 25020, calculated from amino acid sequence Helicobacter pylori
25066
-
4 * 25066, MALDI TOF mass spectrometry Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori Q5UVJ4
-
-
Helicobacter pylori SS1 Q5UVJ4
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel chelating column chromatography, GSH-Sepharose column chromatography, and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
Helicobacter pylori dTMP + tetrahydrofolate + FAD
-
?
5,10-methylenetetrahydrofolate + dUMP + FADH2
-
Helicobacter pylori SS1 dTMP + tetrahydrofolate + FAD
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 25000, SDS-PAGE Helicobacter pylori
homotetramer 4 * 25020, calculated from amino acid sequence Helicobacter pylori
homotetramer 4 * 25066, MALDI TOF mass spectrometry Helicobacter pylori

Synonyms

Synonyms Comment Organism
thymidylate synthase X
-
Helicobacter pylori
ThyX
-
Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Helicobacter pylori

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5
-
Helicobacter pylori

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme is bound to 4 FAD molecules Helicobacter pylori